細菌が新たな遺伝子活性化メカニズムを発見（Bacteria Invent Another Way to Activate Genes）

2026-03-25 コロンビア大学

＜関連情報＞

• https://www.cuimc.columbia.edu/news/bacteria-invent-another-way-turn-genes
• https://www.nature.com/articles/s41586-026-10166-7

転用されたCRISPR–Cas12f相同遺伝子がRNA誘導型転写を促進する Exapted CRISPR–Cas12f homologues drive RNA-guided transcription

Florian T. Hoffmann,Tanner Wiegand,Adriana I. Palmieri,Juniper Glass-Klaiber,Renjian Xiao,Stephen Tang,Hoang C. Le,Chance Meers,George D. Lampe,Leifu Chang & Samuel H. Sternberg
Nature  Published:04 March 2026
DOI:https://doi.org/10.1038/s41586-026-10166-7

Abstract

Bacterial transcription initiation is a tightly regulated process that canonically relies on sequence-specific promoter recognition by dedicated sigma (σ) factors, leading to functional DNA engagement by RNA polymerase (RNAP)¹. Although the seven σ factors in Escherichia coli have been extensively characterized², Bacteroidetes species encode dozens of specialized, extracytoplasmic function σ factors (σ^E) whose precise roles are unknown, pointing to additional layers of regulatory potential³. Here we uncover a mechanism of RNA-guided gene activation involving the coordinated action of σ^E factor in complex with nuclease-dead Cas12f (dCas12f). We screened a large set of genetically linked dCas12f and σ^E homologues in E. coli using RNA and chromatin immunoprecipitation experiments, revealing systems that exhibit robust guide RNA enrichment and DNA target binding with a minimal 5′-G target-adjacent motif. Recruitment of σ^E was dependent on dCas12f and guide RNA, suggesting direct protein–protein interactions, and co-expression experiments demonstrated that the dCas12f–gRNA–σ^E ternary complex was competent for programmable recruitment of the RNAP holoenzyme. Remarkably, dCas12f–RNA–σ^E complexes drove potent gene expression in the absence of any requisite promoter motifs, with de novo transcription start sites defined exclusively by the relative distance from the dCas12f-mediated R-loop. Our findings highlight a new paradigm of RNA-guided transcription that embodies natural features reminiscent of CRISPR activation (CRISPRa) technology^4,5.