2022-12-08 オランダ・デルフト工科大学(TUDelft)
この3Dエンジニアリングされたグリオーマ足場は、脳腫瘍のメカノバイオロジー研究および薬剤スクリーニング研究のための有望なツールとなります。この成果は、『Small』誌に掲載されました。
<関連情報>
- https://www.tudelft.nl/en/2022/3me/news/nastaran-barin-has-developed-a-promising-tool-for-studying-brain-cancer-mechanobiology
- https://onlinelibrary.wiley.com/doi/10.1002/smll.202204485
3D-Engineeredスキャフォールドによる患者由来神経膠腫細胞の微小管と上皮成長因子受容体の局在の研究 3D-Engineered Scaffolds to Study Microtubes and Localization of Epidermal Growth Factor Receptor in Patient-Derived Glioma Cells
Nastaran Barin,Hayri E. Balcioglu,Iris de Heer,Maurice de Wit,Martine L. M. Lamfers,Martin E. van Royen,Pim J. French,Angelo Accardo
Small Published: 07 October 2022
DOI:https://doi.org/10.1002/smll.202204485
Abstract
A major obstacle in glioma research is the lack of in vitro models that can retain cellular features of glioma cells in vivo. To overcome this limitation, a 3D-engineered scaffold, fabricated by two-photon polymerization, is developed as a cell culture model system to study patient-derived glioma cells. Scanning electron microscopy, (live cell) confocal microscopy, and immunohistochemistry are employed to assess the 3D model with respect to scaffold colonization, cellular morphology, and epidermal growth factor receptor localization. Both glioma patient-derived cells and established cell lines successfully colonize the scaffolds. Compared to conventional 2D cell cultures, the 3D-engineered scaffolds more closely resemble in vivo glioma cellular features and allow better monitoring of individual cells, cellular protrusions, and intracellular trafficking. Furthermore, less random cell motility and increased stability of cellular networks is observed for cells cultured on the scaffolds. The 3D-engineered glioma scaffolds therefore represent a promising tool for studying brain cancer mechanobiology as well as for drug screening studies.
