2023-01-17 オークリッジ国立研究所(ORNL)
Hybrid poplar trees such as these shown in an ORNL greenhouse were engineered with the REVEILLE1 gene to delay dormancy and produce more biomass. The research was led by the Center for Bioenergy Innovation at ORNL with the Joint Genome Institute, Brookhaven National Laboratory, the HudsonAlpha Institute for Biotechnology, the University of Connecticut and other partners. Credit: Genevieve Martin/ORNL, U.S. Dept. of Energy
リュウゼツランからメッセンジャーRNAを解読し、休眠と発芽の両方を制御するREVEILLE1遺伝子を発見した。この遺伝子を組み込んだポプラは、温帯地域で生育期間を2～3カ月延長できる可能性があることがわかった。
また、REVEILLE1遺伝子を組み込んだポプラを温室で栽培したところ、バイオマス量が166%増加し、標準のポプラに比べて葉が大きく、茎が太い高木が育った。
ORNLのXiaohan Yang氏は、「概日時計が明暗に対応するように、REVEILLE遺伝子は植物の休眠と覚醒を制御しています」と述べています。”我々は、この遺伝子を用いて、2回の冬に渡って休眠を抑制することに成功しました。”
<関連情報>
- https://www.ornl.gov/news/agave-gene-delays-poplar-dormancy
- https://academic.oup.com/plphys/advance-article/doi/10.1093/plphys/kiac588/6956333?login=false
リュウゼツランREVEILLE1によるポプラの季節的休眠の開始と解除の制御 Agave REVEILLE1 regulates the onset and release of seasonal dormancy in Populus
Degao Liu, Dan Tang, Meng Xie, Jin Zhang, Longmei Zhai, Jiangping Mao, Chao Luo, Anna Lipzen, Yu Zhang, Emily Savage,Guoliang Yuan, Hao-Bo Guo, Dimiru Tadesse, Rongbin Hu, Sara Jawdy, Hua Cheng, Linling Li, Huseyin Yer, Miranda M Clark, Huayu Sun, Jiyuan Shi, Roshani Budhathoki, Rahul Kumar, Troy Kamuda, Yanjun Li, Christa Pennacchio, Kerrie Barry, Jeremy Schmutz, Rajiv Berry, Wellington Muchero, Jin-Gui Chen, Yi Li, Gerald A Tuskan, Xiaohan Yang
Plant Physiology Published::22 December 2022
DOI:https://doi.org/10.1093/plphys/kiac588
Abstract
Deciduous woody plants like poplar (Populus spp.) have seasonal bud dormancy. It has been challenging to simultaneously delay the onset of bud dormancy in the fall and advance bud break in the spring, as bud dormancy and bud break were thought to be controlled by different genetic factors. Here, we demonstrate that heterologous expression of the REVEILLE1 gene (named AaRVE1) from Agave (Agave americana) not only delays the onset of bud dormancy but also accelerates bud break in poplar in field trials. AaRVE1 heterologous expression increases poplar biomass yield by 166% in the greenhouse. Furthermore, we reveal that heterologous expression of AaRVE1 increases cytokinin contents, represses multiple dormancy-related genes, and up-regulates bud break-related genes, and that AaRVE1 functions as a transcriptional repressor and regulates the activity of the DORMANCY-ASSOCIATED PROTEIN 1 (DRM1) promoter. Our findings demonstrate that AaRVE1 appears to function as a regulator of bud dormancy and bud break, which has important implications for extending the growing season of deciduous trees in frost-free temperate and subtropical regions to increase crop yield.
