2023-06-08 カリフォルニア大学サンディエゴ校(UCSD)
<関連情報>
- https://today.ucsd.edu/story/when-water-temperatures-change-the-molecular-motors-of-cephalopods-do-too
- https://www.cell.com/cell/fulltext/S0092-8674(23)00466-X
頭足類におけるRNAの再コード化により微小管モータータンパク質の機能が調整される RNA recoding in cephalopods tailors microtubule motor protein function
Kavita J. Rangan,Samara L. Reck-Peterson
Cell Published:June 08, 2023
DOI:https://doi.org/10.1016/j.cell.2023.04.032
Highlights
•RNA editing in squid specifies unique kinesin protein variants in different tissues
•Unique kinesin variants are made acutely in response to seawater temperature
•Cold-specific kinesin variants have enhanced single molecule motility in the cold
•Cephalopod editomes can reveal functional substitutions in non-cephalopod proteins
Summary
RNA editing is a widespread epigenetic process that can alter the amino acid sequence of proteins, termed “recoding.” In cephalopods, most transcripts are recoded, and recoding is hypothesized to be an adaptive strategy to generate phenotypic plasticity. However, how animals use RNA recoding dynamically is largely unexplored. We investigated the function of cephalopod RNA recoding in the microtubule motor proteins kinesin and dynein. We found that squid rapidly employ RNA recoding in response to changes in ocean temperature, and kinesin variants generated in cold seawater displayed enhanced motile properties in single-molecule experiments conducted in the cold. We also identified tissue-specific recoded squid kinesin variants that displayed distinct motile properties. Finally, we showed that cephalopod recoding sites can guide the discovery of functional substitutions in non-cephalopod kinesin and dynein. Thus, RNA recoding is a dynamic mechanism that generates phenotypic plasticity in cephalopods and can inform the characterization of conserved non-cephalopod proteins.
◆彼らはRNAの再コードを使用してタンパク質レベルでアミノ酸を変化させ、寒冷水域で細胞内の機能の改善を図っていると述べている。
◆彼らの研究では、オパレッセントイカがRNAの再コードによってキネシンとダイニンという分子モーターを変化させ、寒冷な海水温度下でより効果的に機能することが示された。これにより、頭足類は海水温度の変化に応じてプロテオーム(生物の全てのタンパク質の組み合わせ)を調整できる可能性があり、広範な海洋温度下で生存・繁殖できるのかもしれない。また、RNAの再コードは組織によって異なり、異なる動き特性を持つ新しいキネシンのバリアントを生成することもわかった。