2023-10-25 ペンシルベニア州立大学(PennState)
◆この研究は、検査結果が迅速に得られる診療所でのポピュレーションスクリーニングに適しています。現在の標準となっている核酸検査は高価で時間がかかりますが、この新しいテストは2分でクラミジアと淋病を検出できます。この新しいアプローチは他の感染症の検出にも適用でき、感染症の早期検出と治療を支援します。
<関連情報>
- https://www.psu.edu/news/research/story/first-rapid-tests-chlamydia-gonorrhea-exhibit-100-sensitivity/
- https://onlinelibrary.wiley.com/doi/10.1002/advs.202304009
クラミジア・トラコマティスと淋菌感染症の臨床判定に用いる高特異性一本鎖オリゴヌクレオチドと機能性ナノプローブ Highly-Specific Single-Stranded Oligonucleotides and Functional Nanoprobes for Clinical Determination of Chlamydia Trachomatis and Neisseria Gonorrhoeae Infections
Ketan Dighe, Parikshit Moitra, Nivetha Gunaseelan, Maha Alafeef, Tor Jensen, Carla Rafferty, Dipanjan Pan
Advanced Science Published: 23 October 2023
DOI:https://doi.org/10.1002/advs.202304009
Abstract
Early detection of Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) is the key to controlling the spread of these bacterial infections. An important step in developing biosensors involves identifying reliable sensing probes against specific genetic targets for CT and NG. Here, the authors have designed single-stranded oligonucleotides (ssDNAs) targeting mutually conserved genetic regions of cryptic plasmid and chromosomal DNA of both CT and NG. The 5′- and 3′- ends of these ssDNAs are differentially functionalized with thiol groups and coupled with gold nanoparticles (AuNP) to develop absorbance-based assay. The AuNPs agglomerate selectively in the presence of its target DNA sequence and demonstrate a change in their surface plasmon resonance. The optimized assay is then used to detect both CT and NG DNA extracted from 60 anonymized clinical samples with a clinical sensitivity of ∼100%. The limit of detection of the assays are found to be 7 and 5 copies/µL for CT and NG respectively. Furthermore, it can successfully detect the DNA levels of these two bacteria without the need for DNA extraction and via a lateral flow-based platform. These assays thus hold the potential to be employed in clinics for rapid and efficient monitoring of sexually transmitted infections.
