2023-02-07 インペリアル・カレッジ・ロンドン(ICL)
◆研究では、レスベラトロールの生産に焦点を当て、このシステムが生産を改善し、より安定した酵母株の連携を促進することが示されました。この新技術は、食品やバイオ燃料などの持続可能な生産に微生物コミュニティを活用する可能性を示唆しています。
<関連情報>
- https://www.imperial.ac.uk/news/251334/new-molecular-toolkit-boosts-useful-molecule/
- https://www.nature.com/articles/s41564-023-01596-4
合成酵母コミュニティー工学のためのクロスフィード株の分子ツールキット A molecular toolkit of cross-feeding strains for engineering synthetic yeast communities
Huadong Peng,Alexander P. S. Darlington,Eric J. South,Hao-Hong Chen,Wei Jiang & Rodrigo Ledesma-Amaro
Nature Microbiology Published:07 February 2024
DOI:https://doi.org/10.1038/s41564-023-01596-4
Abstract
Engineered microbial consortia often have enhanced system performance and robustness compared with single-strain biomanufacturing production platforms. However, few tools are available for generating co-cultures of the model and key industrial host Saccharomyces cerevisiae. Here we engineer auxotrophic and overexpression yeast strains that can be used to create co-cultures through exchange of essential metabolites. Using these strains as modules, we engineered two- and three-member consortia using different cross-feeding architectures. Through a combination of ensemble modelling and experimentation, we explored how cellular (for example, metabolite production strength) and environmental (for example, initial population ratio, population density and extracellular supplementation) factors govern population dynamics in these systems. We tested the use of the toolkit in a division of labour biomanufacturing case study and show that it enables enhanced and tuneable antioxidant resveratrol production. We expect this toolkit to become a useful resource for a variety of applications in synthetic ecology and biomanufacturing.
