2024-02-27 スイス連邦工科大学ローザンヌ校(EPFL)
◆EPFLの科学者らは、光力の最小量を使用して個々の細菌とウイルスを捕獲し操作するオンチップの「ナノツイーザー」を開発した。この研究は、抗生物質に頼らない治療法の選択肢として有望であり、またウイルスと宿主の相互作用を理解するための貴重なツールを提供している。
<関連情報>
- https://actu.epfl.ch/news/nanotweezers-accelerate-phage-therapy/
- https://onlinelibrary.wiley.com/doi/full/10.1002/smll.202308814
ラベルフリーバクテリオファージのシングルウイルスレベルでの光トラッピングと高速識別 Optical Trapping and Fast Discrimination of Label-Free Bacteriophages at the Single Virion Level
Nicolas Villa, Enrico Tartari, Simon Glicenstein, Hugues de Villiers de la Noue, Emmanuel Picard, Pierre R. Marcoux, Marc Zelsmann, Grégory Resch, Emmanuel Hadji, Romuald Houdré
Small Published: 28 January 2024
DOI:https://doi.org/10.1002/smll.202308814
Abstract
There is a recent resurgence of interest in phage therapy (the therapeutic use of bacterial viruses) as an approach to eliminating difficult-to-treat infections. However, existing approaches for therapeutic phage selection and virulence testing are time-consuming, host-dependent, and facing reproducibility issues. Here, this study presents an innovative approach wherein integrated resonant photonic crystal (PhC) cavities in silicon are used as optical nanotweezers for probing and manipulating single bacteria and single virions with low optical power. This study demonstrates that these nanocavities differentiate between a bacterium and a phage without labeling or specific surface bioreceptors. Furthermore, by tailoring the spatial extent of the resonant optical mode in the low-index medium, phage distinction across phenotypically distinct phage families is demonstrated. The work paves the road to the implementation of optical nanotweezers in phage therapy protocols.
