2025-04-23 カリフォルニア大学ロサンゼルス校 (UCLA)
Steven Jacobsen Lab/UCLA
Arabidopsis thaliana seedlings turn white when a particular gene is knocked out. UCLA and UC Berkeley scientists used a tiny CRISPR system they developed to modify the mother plant’s DNA, which the white seedlings inherited.
<関連情報>
- https://newsroom.ucla.edu/stories/tiny-crispr-tool-faster-simpler-plant-genome-editing
- https://www.nature.com/articles/s41477-025-01989-9
シロイヌナズナにおけるトランスジーンフリー生殖細胞系列編集のためのRNAガイドゲノムエディターのウイルス送達 Viral delivery of an RNA-guided genome editor for transgene-free germline editing in Arabidopsis
Trevor Weiss,Maris Kamalu,Honglue Shi,Zheng Li,Jasmine Amerasekera,Zhenhui Zhong,Benjamin A. Adler,Michelle M. Song,Kamakshi Vohra,Gabriel Wirnowski,Sidharth Chitkara,Charlie Ambrose,Noah Steinmetz,Ananya Sridharan,Diego Sahagun,Jillian F. Banfield,Jennifer A. Doudna & Steven E. Jacobsen
Nature Plants Published:22 April 2025
DOI:https://doi.org/10.1038/s41477-025-01989-9
Abstract
Genome editing is transforming plant biology by enabling precise DNA modifications. However, delivery of editing systems into plants remains challenging, often requiring slow, genotype-specific methods such as tissue culture or transformation1. Plant viruses, which naturally infect and spread to most tissues, present a promising delivery system for editing reagents. However, many viruses have limited cargo capacities, restricting their ability to carry large CRISPR-Cas systems. Here we engineered tobacco rattle virus (TRV) to carry the compact RNA-guided TnpB enzyme ISYmu1 and its guide RNA. This innovation allowed transgene-free editing of Arabidopsis thaliana in a single step, with edits inherited in the subsequent generation. By overcoming traditional reagent delivery barriers, this approach offers a novel platform for genome editing, which can greatly accelerate plant biotechnology and basic research.
