2025-06-10 マックス・プランク研究所
© MPI of Immunobiology & Epigenetics, Hilgers
<関連情報>
- https://www.mpg.de/24855497/0610-immu-running-circles-in-my-head-153800-x
- https://genesdev.cshlp.org/content/early/2025/06/09/gad.352670.125
ELAVは神経細胞における環状RNAの生合成を仲介する ELAV mediates circular RNA biogenesis in neurons
Carlos Alfonso-Gonzalez,Mengjin Shi,Sakshi Gorey,Sarah Holec,Judit Carrasco,Michael Rauer,Stylianos Tsagkris,Fernando Mateos andValérie Hilgers
Genes & Development Published:June 9, 2025
DOI:10.1101/gad.352670.125
Abstract
Circular RNAs (circRNAs) arise from back-splicing of precursor RNAs and accumulate in the nervous systems of animals, where they are thought to regulate gene expression and synaptic function. Here, we show that neuronal circRNA biosynthesis is mediated by the pan-neuronal RNA-binding protein ELAV. In Drosophila embryos, we characterized the circRNA landscape in normal and elav mutant neurons. We found that neuronal circRNAs are globally (>75%) depleted upon ELAV knockout, and induction of ELAV expression drives ectopic RNA circularization. In brain tissue, ELAV binds to pre-mRNA introns flanking putative circRNAs and decreases efficiency of linear splicing in favor of intron pairing at reverse complementary matches, inducing circularization. Together, our data demonstrate that ELAV directly modulates splicing decisions to generate the neuronal circRNA landscape.
