2026-01-07 京都大学 iPS細胞研究所
図1. iMSCを誘導する5つの発生経路 (PN4, 4回継代後の細胞)
<関連情報>
- https://www.cira.kyoto-u.ac.jp/j/pressrelease/news/260107-130000.html
- https://www.cell.com/iscience/fulltext/S2589-0042(25)02743-9
胚性系統特異的iPSC由来間葉系幹/間質細胞は、異なる形態と固有の機能を示す Embryonic lineage-specific iPSC-derived mesenchymal stem/stromal cells exhibit different morphologies and intrinsic functions
Linh Nguyen ∙ Souta Motoike ∙ Denise Zujur ∙ … ∙ Junya Toguchida ∙ Hidetoshi Sakurai ∙ Makoto Ikeya
iScience Published:December 17, 2025
DOI:https://doi.org/10.1016/j.isci.2025.114482
Highlights
- iMSCs were differentiated from the same iPSC line via 5 different embryonic origins
- Different iMSC types displayed different intrinsic characteristics
- Somite-derived iMSCs (SM-iMSCs) showed higher osteogenic differentiation potential
- Cranial neural crest-derived iMSCs (cNCC-iMSCs) have more chondrogenic potential
Summary
Mesenchymal stem/stromal cells (MSCs) have great potential in regenerative medicine owing to their multilineage differentiation capacity. However, tissue-derived MSCs (tMSCs) exhibit inconsistent characteristics. Although induced pluripotent stem cell (iPSC)-derived MSCs (iMSCs) are a potential solution, the effect of different embryonic lineages on their properties remains unknown. We generated MSCs from human iPSCs via five lineage-specific routes: cranial neural crest, trunk neural crest, paraxial mesoderm (somite), lateral plate mesoderm, and limb mesenchyme. All types met established MSC criteria yet differed in morphology, proliferation, and differentiation capacity. Somite-, cranial neural crest-, and limb mesenchyme-derived MSCs showed higher osteogenic potential, whereas somite-derived MSCs also showed high chondrogenic potential but were prone to hypertrophy. Limb mesenchyme-derived MSCs showed the highest adipogenic potential. Transcriptomic profiles indicated distinct clusters within iMSCs. Despite variances, a high correlation level existed between iMSCs and tMSCs. Therefore, iMSCs are potential alternatives to tMSCs in regenerative medicine.
