2026-08-05 ジョージア工科大学（Georgia Tech）
＜関連情報＞
- https://news.research.gatech.edu/feature/immune-aging
- https://www.cell.com/cell-biomaterials/fulltext/S3050-5623(26)00170-4
ナノワイヤーを介したmiRNA送達は、老化したT細胞の機能的応答を回復させる miRNA delivery via nanowires restores functional responses in aged T cells
Zhonghao Dai ∙ Shaylyn Grier ∙ Zhe Zhong ∙ … ∙ Jean L. Koff ∙ Andrés J. García ∙ Ankur Singh
Cell Biomaterials Published:July 3, 2026
DOI:https://doi.org/10.1016/j.celbio.2026.100514
Highlights
- Nanowire enables efficient delivery of miRNAs into resting aged CD8+ T cells
- Multiplexed miRNA delivery enhances activation and effector responses in T cells
- Ex vivo miRNA-programmed aged T cells exhibit enhanced effector priming in vivo
- Multiplexed miRNA enhances T cell function of healthy and cancer aged donors
Summary
Aging reshapes CD8+ T cell immunity by depleting naive populations, narrowing repertoire diversity, and promoting dysfunctional states, thereby weakening responses to infection or vaccination. This study presents a polyethyleneimine (PEI)-functionalized silicon nanowire platform for high-viability, non-viral delivery of single or multiplexed microRNAs (miRNAs) into resting aged CD8+ T cells. Nanowires enable transient modulation of transcriptional programs governing T cell activation, metabolism, proliferation, and differentiation. Delivering a single miRNA influences early activation and differentiation stages, while multiplexed miRNA delivery leads to a broader boost in activation responses, effector functions, metabolic health, and cell proliferation. In vivo, ex vivo-treated aged CD8+ T cells exhibit enhanced early effector priming following antigen exposure. Similarly, in aged human CD8+ T cells, multiplexed miRNA enhances activation and cytotoxic responses and sustains an effector-primed state during early activation and expansion. This method consistently improves function across donors aged from healthy individuals to those with current or past cancer.
