人の皮膚は、三次喫煙や電子タバコの吸い殻にさらされることでダメージを受ける可能性がある(Human skin can be damaged by exposure to thirdhand smoke and electronic cigarette spills)

比較的短時間の被ばくで損傷を引き起こすことが、カリフォルニア大学リバーサイド校の研究で明らかになった A relatively short exposure is sufficient to cause the damage, UC Riverside study finds

2022-06-08 カリフォルニア大学リバーサイド校(UCR)

<関連情報>

• https://news.ucr.edu/articles/2022/06/08/human-skin-can-be-damaged-exposure-thirdhand-smoke-and-electronic-cigarette
• https://www.mdpi.com/2073-4433/13/5/810

EpiDermTM器官型組織およびケラチノサイト単層におけるニコチンの複数の生物学的過程への影響 Nicotine Affects Multiple Biological Processes in EpiDermTM Organotypic Tissues and Keratinocyte Monolayers

Giovanna L. Pozuelos,Matine Rubin,Samantha Vargas,Erik Ramirez,Dhiresh Bandaru,Jihui Sha,James Wohlschlegel and Prue Talbot
Atmosphere  Published: 16 May 2022
DOI:https://doi.org/10.3390/atmos13050810

Abstract

Dermal exposure to nicotine is common due to the widespread use of tobacco products. Here, we assessed the effects of nicotine at concentrations found in thirdhand smoke (THS) contaminated environments and electronic cigarette (EC) spills or leaks on a 3D human skin model (EpiDerm^TM) and on submerged keratinocyte cultures. Air liquid interface treatment of EpiDerm^TM with 10 or 400 μg/mL of nicotine for 24 h followed by proteomics analysis showed altered pathways related to inflammation, protein synthesis, cell–cell adhesion, apoptosis, and mitochondrial function. Submerged cultured keratinocytes were used to validate the proteomics data and further characterize the response of skin cells to nicotine. Mitochondrial phenotype changed from networked to punctate in keratinocytes treated with 10 or 400 μg/mL of nicotine for 48 h and 24 h, respectively. After 72 h, all concentrations of nicotine caused a significant decrease in the networked phenotype. In Western blots, keratinocytes exposed to 400 μg/mL of nicotine had a significant decrease in mitofusin 2, while mitofusin 1 decreased after 72 h. The shift from networked to punctate mitochondria correlated with a decrease in mitofusin 1/2, a protein needed to establish and maintain the networked phenotype. Mitochondrial changes were reversible after a 24 h recovery period. Peroxisomes exposed to 400 μg/mL of nicotine for 24 h became enlarged and were fewer in number. Nicotine concentrations in THS and EC spills altered the proteome profile in EpiDerm^TM and damaged organelles including mitochondria and peroxisomes, which are involved in ROS homeostasis. These changes may exacerbate skin infections, inhibit wound healing, and cause oxidative damage to cells in the skin.