2023-06-06 シンガポール国立大学(NUS)
◆この技術は、細胞の表面タンパク質を利用して分泌されたタンパク質を捕捉し、個々の細胞の機能と関連付けることができます。免疫細胞の応答を単一細胞レベルで研究することが可能であり、新たな治療戦略の開発や疾患に関連するタンパク質の異常分泌を解明する上で有望なツールです。今後はさらなる応用の拡大が期待されています。
<関連情報>
- https://news.nus.edu.sg/powerful-tool-to-gather-data-on-immune-response-at-single-cell-level/
- https://www.nature.com/articles/s41592-023-01841-y
単一細胞のタンパク質分泌をシーケンサーで時間分解評価 Time-resolved assessment of single-cell protein secretion by sequencing
Tongjin Wu,Howard John Womersley,Jiehao Ray Wang,Jonathan Scolnick & Lih Feng Cheow
Nature Methods Published:10 April 2023
DOI:https://doi.org/10.1038/s41592-023-01841-y
Abstract
Secreted proteins play critical roles in cellular communication. Methods enabling concurrent measurement of cellular protein secretion, phenotypes and transcriptomes are still unavailable. Here we describe time-resolved assessment of protein secretion from single cells by sequencing (TRAPS-seq). Released proteins are trapped onto the cell surface and probed by oligonucleotide-barcoded antibodies before being simultaneously sequenced with transcriptomes in single cells. We demonstrate that TRAPS-seq helps unravel the phenotypic and transcriptional determinants of the secretion of pleiotropic TH1 cytokines (IFNγ, IL-2 and TNF) in activated T cells. In addition, we show that TRAPS-seq can be used to track the secretion of multiple cytokines over time, uncovering unique molecular signatures that govern the dynamics of single-cell cytokine secretions. Our results revealed that early central memory T cells with CD45RA expression (TCMRA) are important in both the production and maintenance of polyfunctional cytokines. TRAPS-seq presents a unique tool for seamless integration of secretomics measurements with multi-omics profiling in single cells.
