2026-09-16 産業技術総合研究所
＜関連情報＞
- https://www.aist.go.jp/aist_j/press_release/pr2026/pr20260916/pr20260916.html
- https://www.cell.com/molecular-therapy-family/advances/fulltext/S3117-387X(26)00164-3
HSV-1ゲノム維持293細胞に基づく「EASY-HSVシステム」の開発 Development of the “EASY-HSV system” based on HSV-1 genome-maintaining 293 cells
Fumio Maeda ∙ Moeka Nobe ∙ Kenichiro Imai ∙ Shungo Adachi ∙ Tohru Natsume
Molecular Therapy Advances Published:August 31, 2026
DOI:https://doi.org/10.1016/j.omta.2026.201829
Abstract
The herpes simplex virus (HSV) amplicon vector is a promising molecular tool with a large transgene capacity of approximately 150 kb. However, the production of the HSV amplicon vector requires that Vero cells, which have low gene transfection efficiency, be transfected with genes including the HSV genome, which is challenging due to its large size (150 kb). This increases the production cost of the HSV amplicon vector and limits its broader application. Here, we generated HEK293 cells stably maintain the HSV genome as an episomal DNA during cell division (293/HSV cells). Using these cells, we developed an EASY-HSV (efficient and simple high-yield herpes simplex virus) vector system in which 293/HSV cells are transfected with only a plasmid coding gene of interest and a helper plasmid. Our EASY-HSV vector system offers a practical platform that enhances accessibility to HSV amplicon vectors for researchers seeking effective gene delivery methods.
