マイクロチップにより、血液中のコビド抗体を電子的に検出可能(Microchip Can Electronically Detect Covid Antibodies in Just a Drop of Blood)

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2022-09-21 ジョージア工科大学

指を刺して出る一滴の血液。シンプルな電子チップ。そして、スマートフォンに表示される検査結果は、COVID-19感染症やHIV、ライム病などの診断につながる可能性がある。
標準的な実験手法の基本化学を利用しつつ、光学の代わりに電気伝導度を使用して抗体を検出し、感染を示す小型チップを作製した。
研究者チームは、マルチプレックスチップを作った。このため、1つのチップで、たった1滴の血液から複数の感染症をスクリーニングできる可能性がある。また、チップに付着した銀の量から、血液中の抗体のレベルを定量化することも可能である。

<関連情報>

COVID-19バイオマーカーのマルチプレックスポイントオブケア電気検出のためのナノ構造表面上の酵素増幅メタリゼーションの強化 Enhanced Enzymatically Amplified Metallization on Nanostructured Surfaces for Multiplexed Point-of-Care Electrical Detection of COVID-19 Biomarkers

Neda Rafat,Hanhao Zhang,Josiah Rudge,Yoo Na Kim,Sai Preetham Peddireddy,Nabojeet Das,Aniruddh Sarkar
Small  Published: 29 August 2022
DOI:https://doi.org/10.1002/smll.202203309

Abstract

Inexpensive yet sensitive and specific biomarker detection is a critical bottleneck in diagnostics, monitoring, and surveillance of infectious diseases such as COVID-19. Multiplexed detection of several biomarkers can achieve wider diagnostic applicability, accuracy, and ease-of-use, while reducing cost. Current biomarker detection methods often use enzyme-linked immunosorbent assays (ELISA) with optical detection which offers high sensitivity and specificity. However, this is complex, expensive, and limited to detecting only a single analyte at a time. Here, it is found that biomarker-bound enzyme-labeled probes act synergistically with nanostructured catalytic surfaces and can be used to selectively reduce a soluble silver substrate to generate highly dense and conductive, localized surface silver metallization on microelectrode arrays. This enables a sensitive and quantitative, simple, direct electronic readout of biomarker binding without the use of any intermediate optics. Furthermore, the localized and dry-phase stable nature of the metallization enables multiplexed electronic measurement of several biomarkers from a single drop (<10 µL) of sample on a microchip.This method is applied for the multiplexed point-of-care (POC) quantitative detection of multiple COVID-19 antigen-specific antibodies. Combining a simple microchip and an inexpensive, cellphone-interfaced, portable reader, the detection and discrimination of biomarkers of prior infection versus vaccination is demonstrated.

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