2024-02-26 ミシガン大学
<関連情報>
- https://news.umich.edu/when-plants-are-attacked/
- https://www.cell.com/cell/abstract/S0092-8674(23)01404-6
DGK5を介したPAバーストの二重リン酸化が植物免疫における活性酸素を制御する Dual phosphorylation of DGK5-mediated PA burst regulates ROS in plant immunity
Liang Kong,Xiyu Ma,Chao Zhang,…,Teun Munnik,Ping He,Libo Shan
Cell Published:January 19, 2024
DOI:https://doi.org/10.1016/j.cell.2023.12.030
Highlights
•Immune elicitation triggers two distinct phosphorylation patterns of DGK5
•BIK1- and MPK4-mediated DGK5 phosphorylation opposingly regulates PA burst
•PA binds and stabilizes the NADPH oxidase RBOHD in regulating ROS production
•DGK5 is involved in plant PRR- and NLR-mediated immunity
Summary
Phosphatidic acid (PA) and reactive oxygen species (ROS) are crucial cellular messengers mediating diverse signaling processes in metazoans and plants. How PA homeostasis is tightly regulated and intertwined with ROS signaling upon immune elicitation remains elusive. We report here that Arabidopsis diacylglycerol kinase 5 (DGK5) regulates plant pattern-triggered immunity (PTI) and effector-triggered immunity (ETI). The pattern recognition receptor (PRR)-associated kinase BIK1 phosphorylates DGK5 at Ser-506, leading to a rapid PA burst and activation of plant immunity, whereas PRR-activated intracellular MPK4 phosphorylates DGK5 at Thr-446, which subsequently suppresses DGK5 activity and PA production, resulting in attenuated plant immunity. PA binds and stabilizes the NADPH oxidase RESPIRATORY BURST OXIDASE HOMOLOG D (RBOHD), regulating ROS production in plant PTI and ETI, and their potentiation. Our data indicate that distinct phosphorylation of DGK5 by PRR-activated BIK1 and MPK4 balances the homeostasis of cellular PA burst that regulates ROS generation in coordinating two branches of plant immunity.
Graphical abstract