電波で細菌を制御し、命を守ることができる(Radio waves can tune up bacteria to become life-saving medicines)

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2024-02-29 ロイヤルメルボルン工科大学(RMIT)

研究チームは、DNAを挿入するために細菌の細胞壁を開く際に、化学物質や高温ではなく、高周波の無線波を用いた。この手法は穏やかで、多くの細胞がDNAを受け入れ、生き残ることができる。18ギガヘルツの周波数の無線波を使用して、E. coliの細菌細胞壁を一時的に開いて、遺伝子材料を挿入した。この方法は効率的で、91%の細胞が新しいDNAを取り込み、熱ショック法よりも生存率が高い。また、この手法は動物などの真核細胞でも機能することが示された。

<関連情報>

高周波電磁エネルギーを用いたプラスミドDNAの大腸菌への遺伝子組換え Genetic Transformation of Plasmid DNA into Escherichia coli Using High Frequency Electromagnetic Energy

Palalle G. Tharushi Perera, Denver P. Linklater, Zoltan Vilagosh, The Hong Phong Nguyen, Eric Hanssen, Sergey Rubanov, Steve Wanjara, Bari Aadum, Rebecca Alfred, Chaitali Dekiwadia, Saulius Juodkazis, Rodney Croft, and Elena P. Ivanova
Nano Letters  Published:January 9, 2024
DOI:https://doi.org/10.1021/acs.nanolett.3c03464

Abstract

Abstract Image

We present a novel technique of genetic transformation of bacterial cells mediated by high frequency electromagnetic energy (HF EME). Plasmid DNA, pGLO (5.4 kb), was successfully transformed into Escherichia coli JM109 cells after exposure to 18 GHz irradiation at a power density between 5.6 and 30 kW m–2 for 180 s at temperatures ranging from 30 to 40 °C. Transformed bacteria were identified by the expression of green fluorescent protein (GFP) using confocal scanning microscopy (CLSM) and flow cytometry (FC). Approximately 90.7% of HF EME treated viable E. coli cells exhibited uptake of the pGLO plasmid. The interaction of plasmid DNA with bacteria leading to transformation was confirmed by using cryogenic transmission electron microscopy (cryo-TEM). HF EME-induced plasmid DNA transformation was shown to be unique, highly efficient, and cost-effective. HF EME-induced genetic transformation is performed under physiologically friendly conditions in contrast to existing techniques that generate higher temperatures, leading to altered cellular integrity. This technique allows safe delivery of genetic material into bacterial cells, thus providing excellent prospects for applications in microbiome therapeutics and synthetic biology.

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細胞遺伝子工学
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