2024-09-19 カリフォルニア大学リバーサイド校(UCR)
<関連情報>
- https://news.ucr.edu/articles/2024/09/19/stopping-plants-passing-viruses-their-progeny
- https://www.cell.com/cell-host-microbe/abstract/S1931-3128(24)00316-0
抗ウイルスRNA干渉が植物におけるウイルスの垂直感染を抑制する Antiviral RNA interference inhibits virus vertical transmission in plants
Si Liu ∙ Shou-Wei Ding
Cell Host & Microbe Published:September 6, 2024
DOI:https://doi.org/10.1016/j.chom.2024.08.009
Graphical abstract
Highlights
•Antiviral RNAi inhibits seed transmission of cucumber mosaic virus (CMV) in natural host
•Antiviral RNAi confers resistance to seed infection by CMV in A. thaliana
•Immunofluorescence microscopy reveals predominant embryo invasion in developing seed
•CMV 2b protein antagonizes RNAi-mediated immunosuppression of seed transmission
Summary
Known for over a century, seed transmission of plant viruses promotes trans-continental virus dissemination and provides the source of infection to trigger devastating disease epidemics in crops. However, it remains unknown whether there is a genetically defined immune pathway to suppress virus vertical transmission in plants. Here, we demonstrate potent immunosuppression of cucumber mosaic virus (CMV) seed transmission in its natural host Arabidopsis thaliana by antiviral RNA interference (RNAi) pathway. Immunofluorescence microscopy reveals predominant embryo infection at four stages of embryo development. We show that antiviral RNAi confers resistance to seed infection with different genetic requirements and drastically enhanced potency compared with the inhibition of systemic infection of whole plants. Moreover, we detect efficient seed transmission of a mutant CMV lacking its RNAi suppressor gene in mutant plants defective in antiviral RNAi, providing further support for the immunosuppression of seed transmission by antiviral RNAi.
