2025-05-09 九州大学
<関連情報>
- https://www.kyushu-u.ac.jp/ja/researches/view/1259
- https://www.kyushu-u.ac.jp/f/61630/25_0509_01.pdf
- https://www.sciencedirect.com/science/article/abs/pii/S014102292500078X?via%3Dihub
白色腐朽性担子菌Trametes versicolor由来チトクロームP450モノオキシゲナーゼの機能解析:CYP502B15はカジナン型セスキテルペノイドを装飾する Functional analysis of cytochrome P450 monooxygenases from the white-rot basidiomycete Trametes versicolor: CYP502B15 decorates cadinane-type sesquiterpenoids
Hirofumi Ichinose, Megumu Honda, Tsuyoshi Isogai, Chen Chen, Takuya Kitaoka
Enzyme and Microbial Technology Available online: 21 April 2025
DOI:https://doi.org/10.1016/j.enzmictec.2025.110658
Highlights
- Cytochrome P450 monooxygenases from Trametes versicolor.
- Library of cytochrome P450 monooxygenases and their functions.
- Hydroxylation of γ-cadinene and epicubenol by CYP502B15.
Abstract
Cytochrome P450 monooxygenases (P450) play crucial roles in the diversification of secondary metabolic systems in living organisms. Basidiomycetes produce a wide variety of natural products as secondary metabolites, with their relevance to pharmaceutical and agricultural applications garnering significant interest. Thus, the functional characterization of fungal P450s is essential for basic and applied research of basidiomycetes. We herein describe the identification and functional characterization of P450 in the white-rot basidiomycete Trametes versicolor (TvCYP). A total of 182 TvCYP genes were identified in the T. versicolor genome. Moreover, 145 cDNAs encoding possible mature open reading frames were isolated/generated. Furthermore, we constructed a library of TvCYPs using a yeast expression system that allowed for the semi-comprehensive screening of latent TvCYP functions. Notably, we used this library to identify TvCYPs responsible for the metabolism of cadinane-type sesquiterpenoids. CYP502B15 was revealed to have significant activities against γ-cadinene and epicubenol. On the basis of NMR and X-ray crystal structure experiments, CYP502B15 was observed to catalyze the 3α-hydroxylation of γ-cadinene and epicubenol. To the best of our knowledge, this is the first report of the identification of basidiomycetous P450s responsible for the metabolism of cadinane-type sesquiterpenoids.
