2025-09-08 東京大学
DNA 複製因子 DONSON による DNA 複製と中心体複製の連動
<関連情報>
- https://www.u-tokyo.ac.jp/focus/ja/press/z0111_00089.html
- https://www.u-tokyo.ac.jp/content/400270344.pdf
- https://www.nature.com/articles/s41467-025-63002-3
DNA複製機構は二重信号を伝達し、セントロソーム複製における予定外のライセンス付与と実行を防止する The DNA replication machinery transmits dual signals to prevent unscheduled licensing and execution of centrosome duplication
Kyohei Matsuhashi,Kei K. Ito,Kaho Nagai,Akira Sanada,Koki Watanabe,Kasuga Takumi,Atsushi Toyoda,Masamitsu Fukuyama,Shohei Yamamoto,Takumi Chinen,Grant S. Stewart,Shoji Hata & Daiju Kitagawa
Nature communications Published:08 September 2025
DOI:https://doi.org/10.1038/s41467-025-63002-3
Abstract
Copy number control of DNA and centrosomes is essential for accurate genetic inheritance. DNA replication and centrosome duplication have been recognized as parallel key events for cell division. Here, we discover that the DNA replication machinery directly regulates the licensing and execution processes of centrosome duplication to prevent centrosome amplification. We find that the microcephaly protein DONSON couples DNA replication initiation with Cdc6 translocation to centrosomes. The Cdc6 signal prevents the precocious occurrence of centriole disengagement, the licensing step for centrosome duplication. During DNA replication, DONSON inhibits replisome disassembly by interacting with the CMG helicase, maintaining the intrinsic S/G2 checkpoint signal that blocks centriole-to-centrosome conversion, the execution step for centrosome duplication. Disruption of these dual signals causes precocious centrosome duplication and chromosome mis-segregation, observed in DONSON patient cells. Our results reveal that the DNA replication machinery not only duplicates genetic material but also controls the system for its accurate segregation.
