2025-10-02 基礎生物学研究所,広島大学,東京科学大学,琉球大学,情報・システム研究機構,国立遺伝学研究所,日本獣医生命科学大学,徳島大学,東邦大学,中央大学,玉川大学,鳥取大学
Web要約 の発言:
<関連情報>
- https://www.nibb.ac.jp/press/2025/10/02.html
- https://www.cell.com/iscience/fulltext/S2589-0042(25)01796-1
近交系イモリのゲノムは有尾両生類の行動、発達、再生の分子メカニズムを解明する The inbred newt genome unveils molecular mechanisms of behavior, development, and regeneration in urodele amphibians
Yuki Kimura ∙ Miyuki Suzuki ∙ Akinori Okumura ∙ … ∙ Shuji Shigenobu ∙ Toshinori Hayashi ∙ Ken-ichi T. Suzuki
iScience Published:September 24, 2025
DOI:https://doi.org/10.1016/j.isci.2025.113535
Highlights
- A newt-specific satellite DNA was identified and characterized
- A gene repertoire unique to salamander behavior, development, and regeneration
- CNEs exhibit robust functionality even with massive repetitive sequence insertion
- A regeneration-associated cis-element was found in the Shh limb-specific enhancer
Summary
Salamanders provide excellent models for studying vertebrate evolution, development, and regeneration. To further advance the newt as a model organism in biology, we conducted draft genome sequencing of 20 Gb of an inbred newt (Pleurodeles waltl). As part of this study, the Hoxd11–d13 intergenic region is expanded by over 1 Mb owing to the massive insertion of repetitive sequences including newt-specific satellite DNA. Interestingly, Myod and Bmp4, genes that are typically involved in vertebrate development, are absent in salamanders. Co-option of Sodefrin Precursor-like Factor genes, which encode sex pheromone ligands, suggests a diversification of reproductive behavior among salamanders. Moreover, a limb enhancer of Shh, MFCS1/ZRS, retains its function, even though it is positioned approximately 5 Mb away from the promoter. Furthermore, we have identified a functional cis-element potentially associated with limb regeneration in this enhancer. The newt genome yields crucial insights into amphibian evolution, behavior, development, and regeneration.
