バイオベースの化学物質や燃料を生産するための工学的戦略を改善する Improves engineering strategies for producing bio-based chemicals and fuels
2023-08-21 ロードアイランド大学(URI)
◆モデルは実験的な試行せずに多くの設定をシミュレーションでき、新しいエタノール生産戦略を提案し、化石燃料への依存を減らすための一歩として注目されています。
<関連情報>
- https://www.uri.edu/news/2023/08/uri-researchers-paper-adds-to-understanding-around-ethanol-production/
- https://journals.asm.org/doi/10.1128/aem.00563-23
超好熱性古細菌Pyrococcus furiosusのゲノムスケール代謝モデリングを用いてバイオベースエタノール生産の戦略を最適化する Optimizing Strategies for Bio-Based Ethanol Production Using Genome-Scale Metabolic Modeling of the Hyperthermophilic Archaeon, Pyrococcus furiosus
Jason L. Vailionis, Weishu Zhao, Ke Zhang, Dmitry A. Rodionov, Gina L. Lipscomb, Tania N. N. Tanwee, Hailey C. O’Quinn, Ryan G. Bing, Robert M. Kelly, Michael W. W. Adams, Ying Zhang
Applied and Environmental Microbiology Published:8 June 2023
DOI:https://doi.org/10.1128/aem.00563-23
ABSTRACT
A genome-scale metabolic model, encompassing a total of 623 genes, 727 reactions, and 865 metabolites, was developed for Pyrococcus furiosus, an archaeon that grows optimally at 100°C by carbohydrate and peptide fermentation. The model uses subsystem-based genome annotation, along with extensive manual curation of 237 gene-reaction associations including those involved in central carbon metabolism, amino acid metabolism, and energy metabolism. The redox and energy balance of P. furiosus was investigated through random sampling of flux distributions in the model during growth on disaccharides. The core energy balance of the model was shown to depend on high acetate production and the coupling of a sodium-dependent ATP synthase and membrane-bound hydrogenase, which generates a sodium gradient in a ferredoxin-dependent manner, aligning with existing understanding of P. furiosus metabolism. The model was utilized to inform genetic engineering designs that favor the production of ethanol over acetate by implementing an NADPH and CO-dependent energy economy. The P. furiosus model is a powerful tool for understanding the relationship between generation of end products and redox/energy balance at a systems-level that will aid in the design of optimal engineering strategies for production of bio-based chemicals and fuels.
IMPORTANCE The bio-based production of organic chemicals provides a sustainable alternative to fossil-based production in the face of today’s climate challenges. In this work, we present a genome-scale metabolic reconstruction of Pyrococcus furiosus, a well-established platform organism that has been engineered to produce a variety of chemicals and fuels. The metabolic model was used to design optimal engineering strategies to produce ethanol. The redox and energy balance of P. furiosus was examined in detail, which provided useful insights that will guide future engineering designs.
