2025-06-16 理化学研究所,京都産業大学
魚類初期胚において協調的に遺伝子発現を制御する複数種類のヒストン修飾
<関連情報>
- https://www.riken.jp/press/2025/20250616_2/index.html#wrapper
- https://www.nature.com/articles/s41467-025-60246-x
複数の活性型ヒストン修飾の協調的作用がテレオスト胚における接合体ゲノムの活性化を形成する Coordinated action of multiple active histone modifications shapes the zygotic genome activation in teleost embryos
Hiroto S. Fukushima & Hiroyuki Takeda
Nature Communications Published:16 June 2025
DOI:https://doi.org/10.1038/s41467-025-60246-x
Abstract
Zygotic genome activation (ZGA) is a critical developmental milestone characterized by the rapid and simultaneous activation of genome-widely silenced chromatin. Various active histone modifications accumulate upon ZGA and have long been implicated in ZGA. However, their biological relevance still remains unclear. Here, we comprehensively examined the functional significance of active histone modifications and their writers during ZGA in teleost embryos. Our data propose that developmental genes and housekeeping genes are distinctively regulated during ZGA; CBP/P300 activity is required for developmental gene activation, whereas housekeeping genes depend on non-CBP/P300 histone acetylations H3K9ac/H4K16ac/H3K14ac. Moreover, accumulation of H3K4me2/3 is not prerequisite for activation of all types of genes during ZGA, in contrast to previous reports with cell lines. Finally, temporal accumulation of H3.3S31ph greatly enhances CBP/P300 activity specifically at ZGA, ensuring the activation of developmental genes. Our data demonstrate that multiple histone modifications cooperatively shape ZGA-specific gene activation programs in non-mammalian vertebrates.
