2026-08-12 パシフィック・ノースウェスト国立研究所（PNNL）
Researchers characterized how a yeast functions, evaluated its stress tolerance and redox balancing to different carbon sources, and uncovered regulatory networks. (Image by Andrea Starr | Pacific Northwest National Laboratory)
＜関連情報＞
- https://www.pnnl.gov/publications/carbon-source-drives-phenotypic-behavior-lipomyces-starkeyi
- https://www.nature.com/articles/s41598-026-53531-2
炭素源によって駆動される代謝および調節の再構築が、Lipomyces starkeyiの表現型状態を規定する Carbon source–driven metabolic and regulatory remodeling defines phenomic states in Lipomyces starkeyi
Lummy M. O. Monteiro,Xiaolu Li,Kyle R. Pomraning,Jasmin Alvarez,Song Feng,Teresa Lemmon,Marie Swita,Heather Olson,Josie G. Eder,Tong Zhang,Sneha Couvillion,Jason E. McDermott & Jeffrey J. Czajka
Scientific Reports Published:12 August 2026
DOI:https://doi.org/10.1038/s41598-026-53531-2
Abstract
Lipomyces is a genus of oleaginous yeasts with potential for contributing to reliable biomanufacturing supply chains. However, progress in advanced strain designs and engineering efforts are still constrained by a lack of understanding of the underlying molecular drivers of Lipomyces phenotypes. To address this gap, we collected a suite of multi-omic data to dissect how carbon source availability reshapes the metabolic network, lipid allocation, and regulatory architecture of Lipomyces starkeyi. We observed that glucose promotes biosynthetic and proliferative processes supported by abundant energy and carbon intermediates, xylose enhances redox-balancing mechanisms centered on the pentose phosphate pathway, and glycerol activates respiratory metabolism, β-oxidation, and the glyoxylate cycle. Lipid species distributions remained consistent in both nitrogen replete and depleted conditions across the carbon sources, indicating robust production mechanisms. Regulatory protein identification and network analysis revealed glycerol-driven respiratory growth favors regulatory programs integrating stress tolerance, redox balance, and lipid-associated metabolism, whereas xylose growth activates compensatory transcriptional responses aimed at maintaining mitochondrial function. Nitrogen limitation modulates the strength of these responses but does not fundamentally alter their direction, reinforcing carbon source as the dominant driver of regulatory architecture. Taken together, this data enhances the understanding of Lipomyces molecular rearrangements and provides a foundation for further development of predictive phenotypic tools in this genus.
