2026-10-02 理化学研究所,東京都健康長寿医療センター,バイオテック株式会社,東京科学大学,京都大学,いすみ医療センター
検査技術や検査装置、消耗品を含めた国産化に成功（イラスト：高橋 一誠）
＜関連情報＞
- https://www.riken.jp/press/2026/20261002_1/index.html
- https://www.cell.com/cell-reports-physical-science/fulltext/S2666-3864(26)00493-5
COWFISH2A+は、デジタルバイオ分析のための多用途でアクセスしやすいプラットフォームです COWFISH2A+ as a versatile and accessible platform for digital bioanalysis
Tatsuya Iida ∙ Hajime Shinoda ∙ Mami Yoshimura ∙ … ∙ Hiroaki Takeuchi ∙ Masashi Toyoda ∙ Rikiya Watanabe
Cell reports Physical Science Published:October 1, 2026
DOI:https://doi.org/10.1016/j.xcrp.2026.103587
Highlights
- COWFISH2A+ integrates automated digital bioanalysis in a benchtop platform
- Channel-free low-cost microchips support versatile digital bioanalysis
- Digital enzyme assays distinguish ALP isozymes at the single-molecule level
- SATORI achieves attomolar RNA detection and direct swab testing within 20 min
Summary
Digital bioanalysis enables single-molecule detection of biomolecules and holds transformative potential across the life sciences and clinical diagnostics. However, its broader translation remains limited by accessibility and versatility. Here, we present COWFISH2A+, a compact, fully automated device integrating wide-field imaging with a custom dispensing module. Using an open-type microchip, COWFISH2A+ enables diverse assays at low cost through direct liquid deposition. As a proof of concept, we demonstrate two types of digital bioanalysis: a digital enzyme assay and an amplification-free digital genetic assay, SATORI. The digital enzyme assay discriminates alkaline phosphatase, a blood biomarker, at the single-isozyme level with sub-femtomolar sensitivity, while SATORI achieves attomolar-level detection of multiple pathogen-derived genetic targets. Furthermore, to demonstrate the translational potential of SATORI, we directly test 159 clinical specimens, detecting SARS-CoV-2 from nasopharyngeal swabs within ∼20 min with 92% sensitivity and 85% specificity. COWFISH2A+ thus provides an accessible and versatile platform for digital bioanalysis.
