2026-04-23 埼玉医科大学
図1.ノテキシン注射後に生じるマウス下肢筋石灰化の経時的変化
(Kuratani et al., 2026, DOI:10.1371/journal.pone.0346816より改変)
＜関連情報＞
- https://www.saitama-med.ac.jp/news/nid00000807.html
- https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0346816
損傷によって誘発される石灰化筋壊死の生体内マウスモデル In vivo mouse model of calcific myonecrosis induced by injury
Mai Kuratani,Sho Tsukamoto,Ryo Hamai,Kaori Tsuchiya,Osamu Suzuki,Taketo Yamada,Takenobu Katagiri
PLOS One Published: April 22, 2026
DOI:https://doi.org/10.1371/journal.pone.0346816
Abstract
Skeletal muscle is the major tissue that provides a space and source for bone, which is formed by osteoblasts through the secretion of extracellular matrices, such as type I collagen, and the deposition of hydroxyapatite calcium phosphate crystals. In the present study, we injected notexin, a snake venom, into skeletal muscle to induce local injury. Notexin injection caused local ectopic calcification in muscle fibers within a couple of days, which remained for more than 16 months. In contrast to ectopic bone formation, the damaged muscles after injection of notexin contains neither bone marrow cells, osteoblasts, nor osteoclasts. Histological and chemical analyses of the notexin-induced calcified objects revealed the deposition of stable hydroxyapatite crystals in muscle fibers, which was distinct from ectopic bone formation. We conclude that this in vivo mouse model of calcific myonecrosis induced by injury is useful for studying the molecular mechanisms of and potential therapeutics for calcific myonecrosis.
