コートジボワールにおけるトリパノソマ症寄生虫の遺伝的多様性が明らかに(Research by PASET Scholar Jean-Yves Ekra Reveals Genetic Diversity of Trypanosomiasis Parasites in the Côte d’Ivoire)

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2024-04-02 ウースター工科大学(WPI)

新たな研究によると、コートジボワールのサバンナ地域の牛にトリパノソマ症を引き起こす寄生虫の遺伝的変異が分子生物学的なアプローチで明らかにされました。この研究は、PASETスカラーのジャン・イヴ・エクラによって報告され、バイオテクノロジーおよび神経科学の修士号プログラムのディレクターであるジャガン・スリニヴァサン准教授の指導のもと、Pathogens誌で行われました。トリパノソマ症は、ツェツェバエによって伝播されるトリパノソーマによって引き起こされる人畜共通感染症であり、これまでにサブサハラアフリカでの家畜の拡大における主要な障害の1つを表しています。

<関連情報>

コートジボワール北部サバンナ地区の牛に寄生するトリパノソーマの遺伝的多様性を、rRNA、ITS1、gGAPDHという保存されたゲノムシグネチャーを用いて明らかにした。 Genetic Diversity of Trypanosomes Infesting Cattle from Savannah District in North of Côte d’Ivoire Using Conserved Genomic Signatures: rRNA, ITS1 and gGAPDH

Jean-Yves Ekra,Eliakunda Michael Mafie,Edouard K. N’Goran ,Dramane Kaba ,Biégo Guillaume Gragnon and Jagan Srinivasan
Pathogens  Published: 19 March 2024
DOI:https://doi.org/10.3390/pathogens13030262

コートジボワールにおけるトリパノソマ症寄生虫の遺伝的多様性が明らかに(Research by PASET Scholar Jean-Yves Ekra Reveals Genetic Diversity of Trypanosomiasis Parasites in the Côte d’Ivoire)

Abstract

The potential danger to livestock from African animal trypanosomiasis is well known. However, the trypanosome species circulating in cattle and their genetics are poorly understood. After different alignments according to three regions (ITS1, gGAPDH and rRNA gene) of the trypanosome genome, phylogenetic analyses were used to show the genetic diversity of the different species that were circulating in the cattle in three regions (Bagoue, Poro and Tchologo) of Côte d’Ivoire. These analyses were performed by alignment of ITS1; by alignment of partial 18S, ITS1, 5.8S, ITS2 and partial 28S rRNA genes; and by alignment of gGAPDH gene with sequences of Trypanosomes found in GenBank. Three species were identified (T. vivax, T. theileri and T. congolense) in the cattle in the three northern regions of Côte d’Ivoire. T. vivax and T. theileri were the most abundant species in the present study. Contrary to the other primers used in this study, the ITS1 primers were not able to amplify T. theileri. We observed mixed infections between T. theileri and the other two species identified (T. vivax and T. congolense). As far as primers are concerned, in some cases, rRNA was able to identify the same species of trypanosomes that the ITS1 and gGAPDH primers were able to identify. Two main distinct groups of T. theileri complex were identified. The T. congolense and T. vivax strains were close to African strains, such as those from Kenya, Nigeria and Cameroon, unlike the T. theileri strain. Three trypanosome species (T. vivax, T. theileri and T. congolense) circulate in cattle in the Savannah district of Côte d’Ivoire. The genetic diversity of the trypanosome species encountered in this study cannot be classified as intraspecies according to geographical area and breed of cattle they infect.

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