2026-03-27 東京科学大学
図1. 本研究の概略図
＜関連情報＞
- https://www.isct.ac.jp/ja/news/mtvj9wnelsr9#top
- https://onlinelibrary.wiley.com/doi/10.1111/jcpe.70112
外傷性咬合は、マウスの結紮誘発性歯周炎の骨組織における遺伝子発現を変化させることにより、骨吸収を悪化させる Traumatic Occlusion Exacerbates Bone Resorption by Modifying Gene Expression in the Bone Tissue of Ligature-Induced Periodontitis in Mice
Yosuke Tsuchiya, Yujin Ohsugi, Tomomitsu Hirota, Yohei Hayashi, Peiya Lin, Yuta Tsukahara, Takanori Iwata, Sayaka Katagiri
Journal of Clinical Periodontology Published: 26 March 2026
DOI:https://doi.org/10.1111/jcpe.70112
ABSTRACT
Aim
To clarify the molecular mechanisms of occlusal trauma in bone loss through periodontal tissue transcriptome analysis in mice with periodontitis and traumatic occlusion.
Materials and Methods
Ligature-induced periodontitis (Li) and composite resin-induced traumatic occlusion (Tra) mouse models were established (control [Co], Li, Tra and LiTra). Bone resorption was evaluated using micro-computed tomography (micro-CT). RNA-sequencing was conducted on gingiva, bone and periodontal ligament from all groups 3 days post induction. For long-term evaluation, the Co and Tra groups were maintained for 8 weeks and then analysed using micro-CT and qRT-PCR.
Results
Traumatic occlusion alone, sustained for 8 weeks, did not directly induce bone resorption; however, it significantly exacerbated bone resorption in mice with periodontitis. Cytokine–cytokine receptor interactions and Toll-like receptor signalling pathways were up-regulated in LiTra bone tissue. Il11, Il1rl1 and Mmp3, associated with inflammation and bone metabolism, were more highly expressed in the LiTra group than in the Li group. TNF-α signalling via NFκB and inflammatory response gene sets were enriched in the bone tissue of LiTra group.
Conclusions
Traumatic occlusion accelerates bone resorption in ligature-induced periodontitis but does not independently cause significant bone loss. Occlusal trauma enhances the expression of inflammation-related genes, especially in bone with periodontitis.
