2026-05-08 カリフォルニア大学リバーサイド校（UCR）
＜関連情報＞
- https://news.ucr.edu/articles/2026/05/08/tiny-cell-structure-big-role-brain-development
- https://www.cell.com/cell-reports/fulltext/S2211-1247(26)00433-X
近接標識プロテオミクスにより、発達中の終脳全体にわたる放射状グリア繊毛タンパク質の分布が明らかになった Proximity labeling proteomics maps radial glial ciliary proteins across the developing telencephalon
Xiaoliang Liu ∙ Oscar T. Gutierrez ∙ Sabyasachi Baboo ∙ … ∙ Jolene K. Diedrich ∙ John R. Yates, III ∙ Xuecai Ge
Cell Reports Published:May 7, 2026
DOI:https://doi.org/10.1016/j.celrep.2026.117355
Graphical abstract
Highlights
- In vivo proteomics with TurboID transgenic mice maps radial glial ciliary proteins
- Radial glial primary cilia contain translation machinery proteins
- Ciliary components exhibit region-specific variations in the dorsal and ventral telencephalon
- Mechanistic studies on MARCKS and CKAP2L reveal new ciliary roles in neurodevelopment
Summary
Primary cilia in radial glia act as signaling hubs essential for brain development, yet their molecular roles remain poorly understood. Here, using proximity-labeling-mediated in vivo proteomics, we systematically mapped ciliary proteins in the developing telencephalon. Our dataset reveals region-specific ciliary composition across the dorsal and ventral telencephalon. We identified and validated ribosome proteins and translational machinery components in radial glial cilia. Further, we uncovered ciliary roles for neurodevelopmental disorder-associated proteins, including MARCKS, a key regulator of radial glial polarity, and CKAP2L, a protein linked to Filippi syndrome. Functional studies show that MARCKS contributes to ciliogenesis and CKAP2L regulates neurogenesis by modulating Hedgehog signaling. These findings highlight previously unrecognized mechanisms by which primary cilia modulate brain formation. Our in vivo ciliary proteomic dataset provides a unique resource for understanding ciliary functions in brain development and developmental disorders.
