2026-06-22 デューク大学（Duke）
A cross-section through the brain reveals the symmetric architecture of the vascular network (cyan-green) and the tracer highlighting the glymphatic system (red-yellow) along the brain’s surface and drainage routes — imaged through the intact skull with 3D-PAULM.
＜関連情報＞
非侵襲的な全脳イメージングによるグリンパティックダイナミクスの解析 Noninvasive whole-brain imaging of glymphatic dynamics
Nanchao Wang, Xinyuan Yu, Matthew Lowerison, Qinzhe Li, […] , and Junjie Yao
Science Advances Published:17 Jun 2026
DOI:https://doi.org/10.1126/sciadv.aee4926
Abstract
Cerebrospinal fluid circulation through the glymphatic system plays a crucial role in removing metabolic waste from the central nervous system. However, the mechanism underlying the brain-wide glymphatic dynamics is not yet fully understood, in part due to the lack of glymphatic imaging technologies on deep brains. Here, we report a hybrid imaging technology that integrates three-dimensional photoacoustic tomography and ultrasound localization microscopy (3D-PAULM), enhanced by a photoacoustic dye with strong optical absorption in the second near-infrared window (NIR-II). 3D-PAULM allows for continuous, noninvasive, whole-brain imaging in mice through intact skull, providing superresolution mapping of the brain vasculature and highly sensitive tracing of the NIR-II dye in the glymphatic system. Using 3D-PAULM, we investigated the glymphatic function impaired by ischemic stroke, aging, and anesthesia. Our results provide insights into glymphatic transport under various physiological as well as pathological conditions and establish 3D-PAULM as a valuable tool for preclinical glymphatic research.
