2023-11-03 カリフォルニア大学サンタバーバラ校(UCSB)
◆これらの測定は、細胞や組織が圧力のもとでどのように生存し、圧力の調整に問題が生じると疾患が発症するかを理解するのに役立ちます。新しい技術は、浸透圧を生きた組織内で測定し、「見る」ことを可能にし、多くの設定で浸透圧を理解するための重要なツールとなります。
<関連情報>
- https://news.ucsb.edu/2023/021229/researchers-can-now-visualize-osmotic-pressure-living-tissue
- https://www.nature.com/articles/s41467-023-42024-9
生きた胚組織内の浸透圧の現場測定 In situ quantification of osmotic pressure within living embryonic tissues
Antoine Vian,Marie Pochitaloff,Shuo-Ting Yen,Sangwoo Kim,Jennifer Pollock,Yucen Liu,Ellen M. Sletten &Otger Campàs
Nature Communications Published:02 November 2023
DOI:https://doi.org/10.1038/s41467-023-42024-9
Abstract
Mechanics is known to play a fundamental role in many cellular and developmental processes. Beyond active forces and material properties, osmotic pressure is believed to control essential cell and tissue characteristics. However, it remains very challenging to perform in situ and in vivo measurements of osmotic pressure. Here we introduce double emulsion droplet sensors that enable local measurements of osmotic pressure intra- and extra-cellularly within 3D multicellular systems, including living tissues. After generating and calibrating the sensors, we measure the osmotic pressure in blastomeres of early zebrafish embryos as well as in the interstitial fluid between the cells of the blastula by monitoring the size of droplets previously inserted in the embryo. Our results show a balance between intracellular and interstitial osmotic pressures, with values of approximately 0.7 MPa, but a large pressure imbalance between the inside and outside of the embryo. The ability to measure osmotic pressure in 3D multicellular systems, including developing embryos and organoids, will help improve our understanding of its role in fundamental biological processes.
