Nastaran Barinは、脳腫瘍のメカノバイオロジーを研究するための有望なツールを開発した(Nastaran Barin has developed a promising tool for studying brain cancer mechanobiology)

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2022-12-08 オランダ・デルフト工科大学(TUDelft)

 神経膠腫細胞のメカノバイオロジーを理解することは、この脳腫瘍の将来的な治療法のスクリーニングに最も重要である。エラスムス大学精密・マイクロシステム工学部のNastaran BarinとAngelo Accardoは、同大学との共同研究において、二光子重合法により生体模倣3次元構造体を開発し、そこに患者由来の神経膠腫細胞を培養しました。
この3Dエンジニアリングされたグリオーマ足場は、脳腫瘍のメカノバイオロジー研究および薬剤スクリーニング研究のための有望なツールとなります。この成果は、『Small』誌に掲載されました。

<関連情報>

3D-Engineeredスキャフォールドによる患者由来神経膠腫細胞の微小管と上皮成長因子受容体の局在の研究 3D-Engineered Scaffolds to Study Microtubes and Localization of Epidermal Growth Factor Receptor in Patient-Derived Glioma Cells

Nastaran Barin,Hayri E. Balcioglu,Iris de Heer,Maurice de Wit,Martine L. M. Lamfers,Martin E. van Royen,Pim J. French,Angelo Accardo
Small  Published: 07 October 2022
DOI:https://doi.org/10.1002/smll.202204485

Nastaran Barinは、脳腫瘍のメカノバイオロジーを研究するための有望なツールを開発した(Nastaran Barin has developed a promising tool for studying brain cancer mechanobiology)

Abstract

A major obstacle in glioma research is the lack of in vitro models that can retain cellular features of glioma cells in vivo. To overcome this limitation, a 3D-engineered scaffold, fabricated by two-photon polymerization, is developed as a cell culture model system to study patient-derived glioma cells. Scanning electron microscopy, (live cell) confocal microscopy, and immunohistochemistry are employed to assess the 3D model with respect to scaffold colonization, cellular morphology, and epidermal growth factor receptor localization. Both glioma patient-derived cells and established cell lines successfully colonize the scaffolds. Compared to conventional 2D cell cultures, the 3D-engineered scaffolds more closely resemble in vivo glioma cellular features and allow better monitoring of individual cells, cellular protrusions, and intracellular trafficking. Furthermore, less random cell motility and increased stability of cellular networks is observed for cells cultured on the scaffolds. The 3D-engineered glioma scaffolds therefore represent a promising tool for studying brain cancer mechanobiology as well as for drug screening studies.

生物工学一般
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