植物はいかにして遺伝子の指示を上書きする複数の方法を進化させたか(How plants evolved multiple ways to override genetic instructions)

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2024-11-06 ワシントン大学セントルイス校

ワシントン大学セントルイス校の生物学者チームは、植物がDNAメチル化を通じて遺伝子発現を制御する複数のメカニズムを進化させたことを明らかにしました。特に、CMT3とCMT2という2つの酵素が異なるDNA配列(CHGとCHH)にメチル基を付加する役割を持つことを発見しました。この研究は、植物が環境変化に適応するための遺伝子制御の多様性を示しており、将来的には作物の耐性向上など農業分野での応用が期待されます。

<関連情報>

基質特異性とタンパク質の安定性が植物特異的DNAメチル基転移酵素の分岐を促す Substrate specificity and protein stability drive the divergence of plant-specific DNA methyltransferases

Jianjun Jiang, Jia Gwee, Jian Fang, Sarah M. Leichter, […], and Xuehua Zhong
Science Advances  Published:6 Nov 2024
DOI:https://doi.org/10.1126/sciadv.adr2222

植物はいかにして遺伝子の指示を上書きする複数の方法を進化させたか(How plants evolved multiple ways to override genetic instructions)

Abstract

DNA methylation is an important epigenetic mechanism essential for transposon silencing and genome integrity. Across evolution, the substrates of DNA methylation have diversified between kingdoms. In plants, chromomethylase3 (CMT3) and CMT2 mediate CHG and CHH methylation, respectively. However, how these two methyltransferases diverge on substrate specificities during evolution remains unknown. Here, we reveal that CMT2 originates from a duplication of an evolutionarily ancient CMT3 in flowering plants. Lacking a key arginine residue recognizing CHG in CMT2 impairs its CHG methylation activity in most flowering plants. An engineered V1200R mutation empowers CMT2 to restore CHG and CHH methylations in Arabidopsis cmt2cmt3 mutant, testifying a loss-of-function effect for CMT2 during evolution. CMT2 has evolved a long and unstructured amino terminus critical for protein stability, especially under heat stress, and is plastic to tolerate various natural mutations. Together, this study reveals the mechanism of chromomethylase divergence for context-specific DNA methylation in plants and sheds important lights on DNA methylation evolution and function.

細胞遺伝子工学
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