2025-03-27 大阪大学
<関連情報>
- https://www.u-tokyo.ac.jp/focus/ja/press/z0111_00079.html
- https://www.u-tokyo.ac.jp/content/400260699.pdf
- https://pubs.acs.org/doi/10.1021/acs.nanolett.4c06642
細胞内温度測定のための様々な検出モードを持つ炭素量子ドットを用いた蛍光温度計 Fluorescent Thermometers Based on Carbon Quantum Dots with Various Detection Modes for Intracellular Temperature Measurement
Yuki S. Kato,Yukiho Shimazaki,Shunsuke Chuma,Kota Shiraya,Yurina Nakane,Takuma Sugi,Kohki Okabe,Yoshie Harada,and Shingo Sotoma
Nano Letters Published: March 25, 2025
DOI:https://doi.org/10.1021/acs.nanolett.4c06642
Abstract
We report that carbon quantum dots (CQDs) synthesized via a hydrothermal process using anthraquinone derivatives and l-cysteine provide versatile detection modes, making them suitable for various experimental setups. By modification of the precursor structures, these CQDs can function as different types of fluorescent nanothermometers, including those based on fluorescence intensity, ratiometrics, and fluorescence lifetime. Notably, fluorescence lifetime-based CQDs demonstrate robust performance under a wide range of conditions, including variations in pH and ionic strength. The CQDs exhibit low cytotoxicity and high cellular uptake efficiency, enabling wash-free imaging and precise fluorescence lifetime-based temperature measurements at the single-cell level. Furthermore, we successfully measured temperature changes associated with biochemical reactions, including the increase in cellular temperature induced by mitochondrial depolarization. In addition, these fluorescence lifetime-based measurements could be cross-verified using their fluorescence intensity. These findings underscore the potential of CQDs as versatile and minimally invasive tools for nanoscale thermometry in live cells.
