2026-07-06 シンガポール国立大学（NUS）
＜関連情報＞
- https://news.nus.edu.sg/tool-clearer-view-dna-regulated/
- https://www.nature.com/articles/s41467-026-73609-9
qChIP-MSは、ラベルフリー定量プロテオミクスによって局所的なクロマチン組成を明らかにする qChIP-MS reveals the local chromatin composition by label-free quantitative proteomics
Wai Khang Yong,Nurkaiyisah Zaal Anuar,Zi Wayne Sin,Elayne Su Ru Tan,Grishma Rane,Xiaoman Shao,Chai Yeen Goh,Vartika Khanchandani,Vivian L. S. Kuan,Peh Fern Ong,Maya Jeitany,H. Phillip Koeffler,Lih-Wen Deng,Oliver Dreesen,Takaomi Sanda & Dennis Kappei
Nature Communications Published:26 May 2026
DOI:https://doi.org/10.1038/s41467-026-73609-9 Unedited version
Abstract
Chromatin immunoprecipitation (ChIP) has been a cornerstone for epigenetic analyses over the last decades, but even coupled to sequencing approaches (ChIP-seq), it is ultimately limited to one protein at a time. In a complementary effort, we here combine ChIP with label-free quantitative mass spectrometry (qChIP-MS) to interrogate local chromatin compositions. We demonstrate the versatility of our approach at telomeres, with transcription factors, in tissue and by dCas9-mediated locus-specific enrichment.
