2026-09-18 九州大学
＜関連情報＞
- https://www.kyushu-u.ac.jp/ja/researches/view/1576
- https://www.sciencedirect.com/science/article/pii/S0925400526013468
画像誘導による単一細胞分離のための交換可能な膜ポンプチップを組み込んだマイクロピペット Micropipette integrated with exchangeable membrane-pump chips for imaging-guided single-cell isolation
Nariaki Kiyama, Makoto Saito, Yoko Yamanishi, Mai Yamagishi, Yoshitaka Shirasaki, Shinya Sakuma
Sensors and Actuators B: Chemical Available online: 26 August 2026
DOI:https://doi.org/10.1016/j.snb.2026.140767
Highlights
- Micropipette integrated with exchangeable chips was developed.
- Mechanics-based design enabled flow control tailored to cell size and motility.
- Tunable volumetric resolution and millisecond-scale response were achieved.
- Euglena and Volvox were isolated with > 90% viability.
- The micropipette enabled secretion imaging-guided isolation for single-cell RT-qPCR.
Abstract
Micropipetting provides a technological basis for single-cell analysis that preserves cell-specific behavioral information, thereby enabling imaging-guided single-cell isolation. However, broad applicability across diverse cell sizes and motilities remains constrained by the difficulty of achieving a wide controllable-volume range while maintaining fast and precise flow control. Here, we present a piezo-driven micropipette integrated with exchangeable membrane-pump chips that enables selection of the controllable-volume range through chip replacement and provides tunable volumetric control with resolutions of 0.15, 0.60, and 1.50 pL and response times on the order of 10 ms. Using this platform, we isolated individual Euglena gracilis and Volvox carteri with viabilities exceeding 90%. We further combined live-cell imaging of antibody secretion activity, micropipette-based isolation, and downstream RT-qPCR for hybridoma and Jurkat cells. This platform provides a route to link dynamic cellular phenotypes with downstream molecular analysis in the same cell.
