2022-05-10 ノースカロライナ州立大学(NCState)
メタボロミクスとは、動物の代謝を構成するアミノ酸、脂質、糖などの低分子化合物(メタボローム)の研究です。どのような代謝物がどれくらいの量存在するのかを知ることで、動物の食事と健康との関係を知ることができます。
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H-NMR分光法を用いたアフリカサバンナゾウ(Loxodonta africana)全血の管理下における観測可能な代謝物とメタボロームサンプリングプロトコルの検討 Observable Metabolites and Metabolomic Sampling Protocols for Managed African Savanna Elephant (Loxodonta africana) Whole Blood Using H-NMR Spectroscopy
Jordan Wood,David R. Morgan,Kimberly Ange-van Heugten,Maria Serrano,Larry J. Minter,Vivek Fellner and Michael K. Stoskopf
Metabolites Published: 28 April 2022
DOI:https://doi.org/10.3390/metabo12050400
Abstract
We used nuclear magnetic spectroscopy (NMR) to evaluate the metabolomics of heparinized whole blood drawn from six African savanna elephants (Loxodonta africana) maintained on a well characterized diet. Whole blood samples obtained under behavioral restraint, then quickly frozen in liquid nitrogen, were stored at -80 °C until analysis. Frozen samples were thawed under controlled conditions and extracted with methanol and chloroform to separate the polar and non-polar metabolites. We identified 18 polar metabolites and 14 non-polar lipids using one-dimensional (1D) and two-dimensional (2D) NMR spectra. Despite unexpected rouleaux formation in the thawed frozen samples, spectra were consistent among animals and did not vary dramatically with age or the sex of the animal.
