新しい技術により、機能的なヒト組織のバイオプリントをより忠実に行うことが可能に(A New Technique Creates Greater Fidelity in Bioprinting Functional Human Tissues)

UCサンディエゴのエンジニアが、3Dバイオプリンティングの主要な形態である光散乱の問題に挑む UC San Diego engineers take on the light-scattering problem in a leading form of 3D-bioprinting

2023-02-22 カリフォルニア大学サンディエゴ校(UCSD)

<関連情報>

• https://today.ucsd.edu/story/a-new-technique-creates-greater-fidelity-in-bioprinting-functional-human-tissues
• https://www.science.org/doi/10.1126/sciadv.ade7923

高細胞密度・高解像度3Dバイオプリンティングによる血管組織の作製 High cell density and high-resolution 3D bioprinting for fabricating vascularized tissues

Shangting You,Yi Xiang,Henry H. Hwang,David B. Berry ,Wisarut Kiratitanaporn ,Jiaao Guan,Emmie Yao,Min Tang,Zheng Zhong,Xinyue Ma,Daniel Wangpraseurt ,Yazhi Sun,Ting-yu Lu,Shaochen Chen
Science Advances  Published:22 Feb 2023
DOI:https://doi.org/10.1126/sciadv.ade7923

Abstract

Three-dimensional (3D) bioprinting techniques have emerged as the most popular methods to fabricate 3D-engineered tissues; however, there are challenges in simultaneously satisfying the requirements of high cell density (HCD), high cell viability, and fine fabrication resolution. In particular, bioprinting resolution of digital light processing–based 3D bioprinting suffers with increasing bioink cell density due to light scattering. We developed a novel approach to mitigate this scattering-induced deterioration of bioprinting resolution. The inclusion of iodixanol in the bioink enables a 10-fold reduction in light scattering and a substantial improvement in fabrication resolution for bioinks with an HCD. Fifty-micrometer fabrication resolution was achieved for a bioink with 0.1 billion per milliliter cell density. To showcase the potential application in tissue/organ 3D bioprinting, HCD thick tissues with fine vascular networks were fabricated. The tissues were viable in a perfusion culture system, with endothelialization and angiogenesis observed after 14 days of culture.