2026-06-19 長寿医療研究センター研究所
異種間胚盤胞補完法による肝臓の作製
＜関連情報＞
- https://www.ncgg.go.jp/ri/report/20260615.html
- https://www.cell.com/stem-cell-reports/fulltext/S2213-6711(26)00168-2
Osr1ノックアウトマウスモデルにおける異種間胚盤胞補完を介したラット細胞由来腎臓の生成 Rat cell-derived kidney generation via interspecies blastocyst complementation in an Osr1-KO mouse model
Shunsuke Yuri ∙ Ayako Isotani
Stem Cell Reports Published:June 11, 2026
DOI:https://doi.org/10.1016/j.stemcr.2026.102957
Highlights
- rBC analysis defines lineage-specific requirements for kidney formation
- Osr1-KO mice provide a vacant niche for rat kidney generation by BC
- Rat PSCs generate kidneys in mouse hosts under high donor contribution
- Defines key conditions enabling interspecies kidney generation in the Osr1-KO model
Summary
Interspecies blastocyst complementation (BC) provides a promising approach to generate transplantable organs from pluripotent stem cells (PSCs). However, interspecies kidney generation has remained largely unsuccessful, particularly when using rat PSCs in mouse hosts. Here, we investigated multiple renal-deficient models (Sall1-, Ret-, and Osr1-knockouts [KOs]) through reverse BC (rBC) analyses. We identified Osr1-KO embryos as lacking both nephron progenitor and ureteric bud lineages, providing a vacant developmental niche for renal reconstruction. Injection of rat embryonic stem cells (ESCs) into Osr1-KO mouse blastocysts led to robust rat cell contribution and the formation of kidneys predominantly composed of rat in the vacant niche. These findings establish the Osr1-KO model as a permissive platform for interspecies kidney organogenesis and offer mechanistic insight into developmental compatibility underlying xenogeneic organ generation.
