2026-08-07 九州大学
＜関連情報＞
- https://www.kyushu-u.ac.jp/ja/researches/view/1550
- https://pubs.acs.org/langd5/article-abstract/42/29/21614/5211377/Raft-Affinity-and-Membrane-Modulation-of?redirectedFrom=fulltext
蛍光標識脂質を用いた比較分析により明らかになった、ドコサヘキサエン酸含有リン脂質のラフト親和性と膜調節 Raft Affinity and Membrane Modulation of Docosahexaenoic Acid-Containing Phospholipids Revealed by Comparative Analysis Using Fluorescently Labeled Lipids
Kotaro Shimizu;Yo Yano;Masanao Kinoshita;Nobuaki Matsumori
Langmuir Published:July 15, 2026
DOI:https://doi.org/10.1021/acs.langmuir.6c02774
Abstract
Docosahexaenoic acid (DHA, C22:6) is a polyunsaturated fatty acid that is abundant in the brain. It is also present in biological membranes as DHA-containing phospholipids (DHA-PLs). DHA-PLs are known to modulate lipid rafts; however, the underlying molecular mechanism has not been fully elucidated, primarily owing to the lack of methods to analyze the behavior of DHA-PLs in membranes at the molecular level. To address this, we synthesized a fluorescently labeled DHA-PL using our previously developed labeling method that preserves the intrinsic physicochemical behavior of lipids. For comparison, a fluorescent analog of arachidonic acid-containing phospholipids (AA-PLs) was also prepared. Using these fluorescently labeled lipids, we measured their lateral diffusion coefficients and partitioning ratios in phase-separated membranes. The results demonstrate that DHA-PL exhibits a higher affinity for lipid raft-like domains than AA-PL and that its incorporation induces compositional changes and modulates raft-like membrane organization. This study clearly demonstrates how subtle differences in unsaturated acyl chains affect phospholipid behavior in membranes and establishes our labeling technology as a powerful platform for probing the molecular behavior of polyunsaturated phospholipids in membranes.