人工細胞を超音波で踊らせる(Making engineered cells dance to ultrasound)

2023-02-23 カリフォルニア工科大学(Caltech)

<関連情報>

• https://www.caltech.edu/about/news/making-engineered-cells-dance-to-ultrasound
• https://www.science.org/doi/10.1126/sciadv.add9186

遺伝子選択的な細胞音響操作のための生体分子アクチュエーター Biomolecular actuators for genetically selective acoustic manipulation of cells

Di Wu,Diego Baresch ,Colin Cook,Zhichao Ma,Mengtong Duan,Dina Malounda ,David Maresca,Maria P. Abundo,Justin Lee,Shirin Shivaei ,David R. Mittelstein,Tian Qiu,Peer Fischer,Mikhail G. Shapiro
Science Advances  Published:22 Feb 2023
DOI:https://doi.org/10.1126/sciadv.add9186

Abstract

The ability to physically manipulate specific cells is critical for the fields of biomedicine, synthetic biology, and living materials. Ultrasound has the ability to manipulate cells with high spatiotemporal precision via acoustic radiation force (ARF). However, because most cells have similar acoustic properties, this capability is disconnected from cellular genetic programs. Here, we show that gas vesicles (GVs)—a unique class of gas-filled protein nanostructures—can serve as genetically encodable actuators for selective acoustic manipulation. Because of their lower density and higher compressibility relative to water, GVs experience strong ARF with opposite polarity to most other materials. When expressed inside cells, GVs invert the cells’ acoustic contrast and amplify the magnitude of their ARF, allowing the cells to be selectively manipulated with sound waves based on their genotype. GVs provide a direct link between gene expression and acoustomechanical actuation, opening a paradigm for selective cellular control in a broad range of contexts.