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- https://www.riken.jp/press/2026/20260520_1/index.html
- https://www.jstage.jst.go.jp/article/plantbiotechnology/advpub/0/advpub_26.0330b/_article
ã¯ãºïŒ Pueraria montana var. lobata ïŒã«ããããã¯ããªã²ãã³çåæã«é¢äžããã€ãœãã©ãã³6-ããããã·ã©ãŒãŒã®åå®ãš6-ã¡ããã·ã€ãœãã©ãã³ã®å¹ççãªçç£ Identification of an isoflavone 6-hydroxylase involved in tectorigenin biosynthesis in kudzu (Pueraria montana var. lobata) and the efficient production of 6-methoxyisoflavones
Kai Uchida , Masami Yokota Hirai
Plant Biotechnology Published: May 20, 2026
DOI:https://doi.org/10.5511/plantbiotechnology.26.0330b
Abstract
Kudzu (Pueraria montana var. lobata) produces several isoflavones that have beneficial effects in humans. These isoflavones accumulate in a tissue-specific manner, with 5, 7, 4â²-trihydroxy-6-methoxyisoflavone (tectorigenin) and its derivatives accumulating mainly in flowers. Tectorigenin has various beneficial effects on humans and is in high demand, but its biosynthesis has not been elucidated, making large-scale production difficult. In this study, we elucidated the steps in tectorigenin biosynthesis in kudzu by identifying a novel isoflavone 6-hydroxylase and isoflavone O-methyltransferase through a transcriptome analysis and related enzyme assays. Furthermore, using yeast engineered to express genes encoding these enzymes, we successfully produced the 6-methoxyisoflavones tectorigenin, glycitein, and afrormosin from the inexpensive isoflavones genistein, daidzein, and formononetin, respectively. The tectorigenin production rate was 40 mg lâ1 after being cultured for 72 h. These results contribute to large-scale tectorigenin production and to tectorigenin-related research.
