2025-10-27 物質・材料研究機構,名古屋⼤学,岐阜⼤学
図: 細胞に無害な赤色光〜近赤外光を2種類用いて励起することにより、
細胞内でDNAとRNAを同時に見分ける(左)と細胞が老化していくのがわかる(右)
<関連情報>
- https://www.nims.go.jp/press/2025/10/202510270.html
- https://www.nims.go.jp/press/2025/10/mb133t0000004krh-att/202510270.pdf
- https://www.science.org/doi/10.1126/sciadv.adz6633
近赤外デュアルRNA/DNA標的プローブを用いた複数の細胞運命の記録の可視化 Visualizing the chronicle of multiple cell fates using a near-IR dual-RNA/DNA–targeting probe
Linawati Sutrisno, Gary J. Richards, Jack D. Evans, Michio Matsumoto, […] , and Katsuhiko Ariga
Science Advances Published:22 Oct 2025
DOI:https://doi.org/10.1126/sciadv.adz6633
Abstract
Early detection and late-stage cell fate assessment are key factors to develop therapeutic strategies, although current methods cannot capture early responses or distinguish multiple injury states, especially in ultraviolet-visible (UV-vis)–sensitive cells. Here, we introduce a method to simultaneously detect variations in RNA and DNA under near-infrared photoexcitation. Using a pyrazinacene-based probe (TEG8-N14), we unexpectedly achieved discrimination of multiple cell states, including apoptosis, necrosis, necroptosis, and senescence, based on RNA/DNA changes. Specifically, TEG8-N14 selectively stains necrotic cells in live samples, while after fixation, it allows detection of ultraearly senescence in UV-vis–sensitive cells, providing approximately twofold greater informational content than existing RNA or DNA fluorophores. These findings break current imaging barriers by enabling comprehensive visualization of single-cell fate histories without being affected by UV-vis or genetic manipulation.
