2026-09-29 バース大学
＜関連情報＞
- https://www.bath.ac.uk/announcements/fluorescent-sensors-could-help-researchers-see-diabetes-in-action/
- https://advanced.onlinelibrary.wiley.com/doi/10.1002/advs.77481
Celluloおよびインスリン欠乏ゼブラフィッシュにおけるホウ酸ハイブリッドの糖質を介した細胞内取り込み：ライブイメージングと多光子FLIMの応用 Carbohydrate-Mediated Cellular Uptake of Boronic Acid Hybrids In Cellulo and Insulin-Deficient Zebrafish: Live Imaging and Application of Multiphoton FLIM
Haobo Ge, John S. Fossey, David Gonzalez Calatayud, Stephen E. Flower, Sabrina Wang, Hanna Aitessaid, Melita Tvardauskaite, Julia Dudzic, Max Jewell, …
Advanced Science Published: 10 September 2026
DOI:https://doi.org/10.1002/advs.77481
ABSTRACT
Understanding how synthetic molecular probes interact with biological carbohydrates is essential for theranostics design and discovery. We report new supramolecular polysaccharide complexes, comprising simultaneously functionalised boronic acids conjugated to xanthate-based fluorescent tags bound to saccharides such as glucose or β-D-glucan (a natural product from Hordeum Vulgare). These hybrids were investigated in solution and in the cellular milieu using advanced biophysical methods, including multiphoton fluorescence lifetime imaging microscopy (MP-FLIM). A new type of fluorescent hybrid nanoparticle was also generated by conjugating boronic acids to ─Si(OH)2 functionalities on the surface of core–shell Fe3O4@SiO2 nanoparticles. Cellular uptake and distribution were probed using correlated confocal and multiphoton fluorescence microscopy using 2-photon excitation at 910 nm, whereby TCSPC and FLIM provided insight into probe environments, interactions with β-D-glucan, and cellular context. Colocalization studies with a range of standard dyes were carried out to assess the potential of the probes for cellular penetration and targeting cellular organelles, and the nature of the lipophilic linker involved greatly influences the cytoplasmic distribution and facilitates zebrafish uptake. Complementary in vivo investigations using fluorescence stereomicroscopy of zebrafish showed differential uptake and quenching of these complexes throughout the digestive system, dependent on exogenous and endogenous concentrations of carbohydrates, glucose, or β-d-glucan.
