2026-05-29 合肥物質科学研究院（HFIPS）
Schematic diagram of carbon fixation by plasma mutagenesis in microalgae（Image by DUAN Wenxue）
＜関連情報＞
- https://english.hf.cas.cn/nr/rn/202605/t20260529_1160076.html
- https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/cbf.3991
- https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/bit.70071
- https://www.sciencedirect.com/science/article/abs/pii/S0960852425010971
大気中および室温プラズマの最近の進歩は、有望な新しい突然変異誘発技術である Recent progress of atmospheric and room-temperature plasma as a new and promising mutagenesis technology
Dongao Li, Jie Shen, Qiang Ding, Jinyong Wu, Xiangsong Chen
Cell Biochemistry and Function Published: 26 March 2024
DOI:https://doi.org/10.1002/cbf.3991
Abstract
At present, atmospheric and room-temperature plasma (ARTP) is regarded as a new and powerful mutagenesis technology with the advantages of environment-friendliness, operation under mild conditions, and fast mutagenesis speed. Compared with traditional mutagenesis strategies, ARTP is used mainly to change the structure of microbial DNA, enzymes, and proteins through a series of physical, chemical, and electromagnetic effects with the organisms, leading to nucleotide breakage, conversion or inversion, causing various DNA damages, so as to screen out the microbial mutants with better biological characteristics. As a result, in recent years, ARTP mutagenesis and the combination of ARTP with traditional mutagenesis have been widely used in microbiology, showing great potential for application. In this review, the recent progress of ARTP mutagenesis in different application fields and bottlenecks of this technology are systematically summarized, with a view to providing a theoretical basis and technical support for better application. Finally, the outlook of ARTP mutagenesis is presented, and we identify the challenges in the field of microbial mutagenesis by ARTP.
Significance statement
The aim of this review is to analyze the beneficial mutagenic effects induced by ARTP on microbial cells and the related mutagenic mechanisms. Subsequently, bottleneck issues and the recent research progress of ARTP on mutagenesis are systematically summarized, and the application prospect is presented. This review may provide valuable insights and research ideas for future atmospheric and room-temperature plasma mutagenesis of cells.
気液相プラズマによるクロレラ・ソロキニアナの酸化損傷メカニズムの調査 Investigation of Oxidative Damage Mechanism of Chlorella sorokiniana by Gas–Liquid Phase Plasma
Dong-Ao Li, Guang-Hui Xu, Jie Shen, Hong Zhang
Biotechnology and Bioengineering Published: 24 September 2025
DOI:https://doi.org/10.1002/bit.70071
ABSTRACT
This study explores the mechanisms of oxidative damage in Chlorella sorokiniana under continuous gas-liquid phase plasma exposure. The oxidative damage mechanism of C. sorokiniana was revealed through multiple analytical techniques, including scanning electron microscopy (SEM), flow cytometry (FCM), and fluorescence excitation-emission matrix (EEM). Plasma-induced damage to algal cell membranes, causing seepage of photosynthetic pigments and dissolved organic matter; chlorophyll fluorescence changes confirmed damage to the photosynthetic system. Alterations in MDA, intracellular ROS/NO, and antioxidant enzyme activities reflect lipid peroxidation and oxidative stress levels. These results show that plasma induces oxidative damage to algal membranes, intracellular components, and photosystems via reactive oxygen and nitrogen species (RONS). C. sorokiniana activates its antioxidant defense system under stress. This gradual, cumulative damage exerts distinct biological effects at different times. The findings support the precise control of low-temperature plasma for beneficial microorganisms and provide a basis for advancing microalgae transformation technologies.
低温プラズマ突然変異誘発によるクロレラ・ソロキニアナ変異株の育種による効率的な炭素隔離 Low-temperature plasma mutagenesis breeding of Chlorella sorokiniana mutant strains for efficient carbon sequestration
Dong-Ao Li, Hong Zhang, Guang-Hui Xu, Jie Shen, Qing Huang
Bioresource Technology Available online: 7 August 2025
DOI:https://doi.org/10.1016/j.biortech.2025.133130
Highlights
- Mutant strains were mutagenized and screened using low-temperature plasma.
- Characteristics of genetic changes in mutant strains verified by RAPD analysis.
- Photosynthetic performance was enhanced and high light acclimatization increased.
- Rubisco activity, CA activity and NADPH content were all increased.
- Carbon and nitrogen metabolism pathways are enhanced.
Abstract
In this study, low-temperature plasma was used to achieve mutagenesis of Chlorella sorokiniana and two mutants with good traits were successfully obtained after several rounds of screening. The results showed that the biomass of the mutants was increased by 15.31% and 19.79%, respectively, compared with the wild-type strain, and the lipid content was enhanced by 8.90% and 2.31%, respectively. The photosynthetic performance, enzyme activities, NADPH content, carbon fixation rate, and nitrogen assimilation efficiency were all improved. Transcriptome sequencing analysis indicated that mutations in genes associated with the central carbon metabolism pathway underwent modulation as a major factor influencing the physiological performance of mutants. Therefore, this study demonstrates that low-temperature plasma may be used as a promising and efficient tool for acquiring microalgae mutants with desired high value-added products and improved carbon fixation rate.
