2026-07-02 カリフォルニア大学サンディエゴ校（UCSD）
＜関連情報＞
- https://today.ucsd.edu/story/subtle-difference-bacteria-survival-strategies
- https://www.science.org/doi/10.1126/science.aeb6410
細菌におけるグローバル代謝フラックスとプロテオーム分配の分離 Decoupling of global metabolic flux and proteome partitioning in bacteria
Ryan Thiermann, Jin Yang, Aniket Zodage, Fukang She, […] , and Suckjoon Jun
Science Published:18 Jun 2026
DOI:https://doi.org/10.1126/science.aeb6410
GTP-based control of proteome allocation and metabolic flux in bacteria.
Abstract
Bacteria regulate homeostatic growth by adjusting proteome composition. In Escherichia coli, this coordination is mediated by guanosine tetraphosphate and pentaphosphate, collectively termed (p)ppGpp, which couple amino acid supply with ribsosome production. We identified a distinct architecture in Bacillus subtilis, in which guanosine triphosphate (GTP), not (p)ppGpp, controls proteome allocation. Translational inhibition resulted in GTP depletion and suppressed amino acid biosynthesis through feedback inhibition without altering ribosome abundance, establishing a regulated decoupling between total amino acid flux and proteome composition, with flux deviating from proteome-based predictions. By artificially adjusting GTP concentrations, we recoupled flux and proteome, restoring growth to maximal amounts. The regulated suboptimality enables a trade-off to balance growth and stress resilience. Similar GTP-based strategies were present in other Firmicute species, indicating possible evolutionary conservation. Proteome composition and metabolic flux have distinct regulatory layers in some bacteria.
