2026-08-25 パシフィック・ノースウェスト国立研究所(PNNL)
＜関連情報＞
- https://www.pnnl.gov/publications/molecular-environment-composition-shapes-substrate-utilization-dynamics-influencing
- https://journals.asm.org/doi/10.1128/msystems.00760-26
真菌の滲出液に着想を得た基質混合物は、 Pseudomonas putidaにおいて新たな増殖表現型と代謝応答を引き起こす A fungal exudate-inspired substrate mixture elicits an emergent growth phenotype and metabolic responses in Pseudomonas putida
Natalie Sadler, Elise Van Fossen, Grace Black, Vanessa Paurus, Nathalie Munoz, Yuqian Gao, Isaac Kwame Attah, Damon Leach, Joonhoon Kim, Sneha Couvillion
mSystems Published:24 July 2026
DOI:https://doi.org/10.1128/msystems.00760-26
ABSTRACT
Microbes in natural environments often encounter diverse mixtures of organic compounds, yet how mixed substrate environments and their molecular composition shape microbial phenotypes remains understudied. Here, we examined how a defined fungal exudate mimic (FEM) mixture influences growth and metabolism in Pseudomonas putida KT2440 compared to individual substrates matched for total carbon and nitrogen. Growth on FEM initiated 2 h earlier than growth on glucose alone and exhibited both the lowest lag and shortest time to maximum biomass compared to individual substrates. Fructose was the only individual substrate that supported significantly higher maximum biomass than FEM, but exhibited a nearly 15-fold longer lag phase. Gas chromatography mass spectrometry analysis revealed dynamic temporal patterns of substrate utilization within the FEM mixture, with early preferential utilization of malate, followed by overlapping utilization of multiple substrates between 3 and 8 h. By integrating growth and substrate uptake kinetics with genome-scale metabolic modeling and validating model-predicted pathway activity using temporal proteomics, we show that experimentally constrained model predictions accurately captured substrate utilization dynamics across multiple FEM concentrations, and predicted temporal shifts in the dominant substrates supporting growth. Through this integrative experimental-modeling approach, we demonstrate that the mixed substrate FEM environment elicits an emergent growth phenotype characterized by the lowest lag, shortest time to maximum biomass, and relatively high maximum biomass in Pseudomonas putida, a combination of traits not simultaneously reproduced by any individual substrate.
