2023-08-16 ペンシルベニア州立大学(PennState)
◆白いボタンキノコに特定された新しい化合物は、げっ歯動物(マウス、ブタ、人間を含む)に存在するアリールヒドロカーボン受容体(AHR)を活性化することが分かりました。AHRは腸の健康に重要な役割を果たし、活性化されると、腸内の発がん物質であるアリールヒドロカーボンを解毒する細胞応答を引き起こすことがあります。この研究は、食品の各化学成分が全体の健康に果たす役割を研究する重要性を強調しています。
<関連情報>
- https://www.psu.edu/news/agricultural-sciences/story/natural-compound-white-button-mushrooms-could-benefit-animal-human/
- https://www.sciencedirect.com/science/article/pii/S1756464623002025
モレキュラーネットワーキングにより、シロボタンマッシュルーム(Agaricus bisporus)由来のAHR調節ベンゾチアゾールが同定される Molecular networking identifies an AHR-modulating benzothiazole from white button mushrooms (Agaricus bisporus)
Xiaoling Chen, Andrew D. Patterson, Gary H. Perdew, Iain A. Murray, Joshua J. Kellogg
Journal of Functional Foods Available online: 7 June 2023
DOI:https://doi.org/10.1016/j.jff.2023.105602
Highlights
•Agaricus bisporus may mediate gut health via the aryl hydrocarbon receptor (AHR).
•Untargeted metabolomics-molecular networking predicted novel mushroom AHR modulator.
•Increased efficiency in identifying potential AHR bioactives.
•2-amino-4-methyl-benzothiazole is a novel AHR modulator from white button mushrooms.
Abstract
Diet-derived aryl hydrocarbon receptor (AHR) ligands have potential to maintain gut health. However, among the myriad bioactive compounds from foods, identifying novel functional ligands which would significantly impact gastrointestinal health is a challenge. In this study, a novel AHR modulator is predicted, identified, and characterized in the white button mushroom (Agaricus bisporus). Using a molecular networking approach, a methylated analog to benzothiazole was indicated in white button mushrooms, which was subsequently isolated and identified as 2-amino-4-methyl-benzothiazole(2A4). Cell-based AHR transcriptional assays revealed that 2-amino-4-methyl-benzothiazole possesses agonistic activity and upregulated CYP1A1 expression. This contrasts with previous findings that whole white button mushroom extract has overall antagonistic activity in vivo, underscoring the importance of studying the roles each chemical component plays in a whole food. The findings suggest that 2-amino-4-methyl-benzothiazole is a previously unidentified AHR modulator from white button mushroom and demonstrate that molecular networking has potential to identify novel receptor modulators from natural products.
