バイオ水素生成とCO2回収を両立する発酵システムを開発（Wollastonite-Enabled Fermentation System Boosts Biohydrogen Yield While Capturing Carbon Dioxide）

2026-05-08 中国科学院（CAS）

＜関連情報＞

• https://english.cas.cn/newsroom/research-news/202605/t20260508_1158608.shtml
• https://www.sciencedirect.com/science/article/abs/pii/S1385894726036259

負の炭素バイオ水素を目指して：発酵促進とCO2隔離のための二重機能ウォラストナイト戦略 Towards negative-carbon biohydrogen: A dual-function wollastonite strategy for enhanced fermentation and CO2 sequestration

Weiming Li, Can Jin, Huida Duan, Fangcao Li, Zhangxun Huang, Haodong Zhao, Xiaoman Yu, Yimei Xi, Xiangfeng Zeng, Yongfeng Jia, Chi Cheng

Chemical Engineering Journal  Available online: 13 April 2026

DOI:https://doi.org/10.1016/j.cej.2026.176164

Graphical abstract

Highlights

• Wollastonite as a pH buffer boosts bio-hydrogen production rate and yield.
• Metabolic flux shifts from lactate to acetate, enhancing H₂ production.
• In-situ CO₂ sequestration is achieved via wollastonite-induced mineralization.
• A two-stage strategy co-optimizes H₂ production and CO₂ sequestration.
• LCA confirms a 37% process energy saving and lower environmental impacts.

Abstract

Dark fermentation for biohydrogen requires continuous alkaline dosing to control process acidification and expensive separation units to remove co-produced CO₂, resulting in high operational costs and energy consumption. Here, we introduce wollastonite as a dual-function agent to simultaneously enhance H₂ yield and capture CO₂. An optimal dosage of 10 g/L was identified, which shortened the lag phase from 23.13 to 12.38 h and increased the hydrogen yield from 158.11 ± 3.44 mL/g glucose-consumed to 210.75 ± 15.87 mL/g glucose-consumed. Mechanistically, wollastonite buffered the system pH, steering metabolic flux away from lactate towards acetate synthesis by enriching Clostridium and suppressing Lactobacillus. Wollastonite also enabled in-situ CO₂ sequestration by precipitating it as CaCO₃. However, maximal CO₂ capture was achieved at a higher dosage (≥15 g/L), which passively reached the required neutral pH but compromised the hydrogen yield. This created a conflict with the optimal 10 g/L dosage for hydrogenesis. To prioritize the primary goal of green hydrogen production, a two-stage strategy was developed. This approach first uses the optimal 10 g/L dosage for maximal fermentation, followed by a post-fermentation pH adjustment from 6.54 ± 0.18 to 7.0 to induce carbonation. The optimized process successfully sequestered 0.49 ± 0.05 of CO₂ per liter of medium, thereby increasing the H₂ content in the final biogas to a high of 58.2 ± 1.1%. Finally, a life cycle assessment (LCA) validated the environmental superiority of this strategy, confirming a significantly lower Global Warming Potential (GWP) for the entire process. This work thus provides a proof-of-concept for a pragmatic strategy that co-optimizes hydrogen production and carbon capture in a single, sustainable biorefinery process.