2026-06-05 京éœå€§åŠ
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- https://www.kyoto-u.ac.jp/ja/research-news/2026-06-05-0
- https://www.sciencedirect.com/science/article/abs/pii/S1567539426001003
ã®é žè±æ°ŽçŽ é µçŽ ã®çµæããžã¢ãã©ã©ãŒãŒãµããŠãããã«ããçŽæ¥é»å移ååNADHåçãããŒãªã¢ã¯ã¿ã®æ§ç¯ Direct electron transfer-type NADH regeneration flow reactor with recombinant diaphorase subunit of formate dehydrogenase 1
Reiji Kinosada, Sachika Takebe, Taiki Adachi, Shinichi Oide, Yuki Kitazumi, Osamu Shirai, Keisei Sowa
Bioelectrochemistry Available online: 30 April 2026
DOI:https://doi.org/10.1016/j.bioelechem.2026.109314
Highlights
- Diaphorase subunit of formate dehydrogenase 1 (EcFoDH1B) was expressed in E. coli.
- Direct electron transfer-type bioelectrocatalysis with EcFoDH1B was investigated.
- NADH regeneration flow reactor with EcFoDH1B was constructed.
- The flow reactor achieves high TOF and Faradaic efficiency under low overpotential.
Abstract
Nicotinamide adenine dinucleotide redox couple (NAD+/NADH) plays important roles in vivo as a redox cofactor, making NADH regeneration systems valuable to the biochemical industry. Among various methods, electrochemical NADH regeneration employing direct electron transfer (DET)-type bioelectrocatalysis offers low overpotentials and high specificity without the need for mediators. A recombinant β subunit (diaphorase subunit) of formate dehydrogenase 1 (FoDH1B) from Methylorubrum extorquens AM1 has reportedly shown DET-type activity in NAD+/NADH interconversion. In this study, a heterologous expression system for FoDH1B in Escherichia coli (EcFoDH1B) was constructed to improve protein expression efficiency, and its electrochemical properties were elucidated, exhibiting 10-fold higher DET-type activity than that expressed in M. extorquens AM1. In addition, appropriate conditions for the DET-type reaction were investigated. Furthermore, a bioelectrochemical NADH regeneration flow reactor was constructed using an EcFoDH1B-modified carbon felt electrode, achieving a turnover frequency of 10,000 hâ1 and a Faradaic efficiency over 90% at a low overpotential (0.10 V).
